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Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof
We present a different strategy for synthesizing the Au-γ-Fe(2)O(3) bifunctional nanoparticle by using a larger (50 nm) Au nanoparticle as the core surrounded by smaller (10 nm) γ-Fe(2)O(3) nanoparticles. The synthesis of the composite nanoparticles is quite facile based on a simple redox process wh...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423036/ https://www.ncbi.nlm.nih.gov/pubmed/22759882 http://dx.doi.org/10.1186/1556-276X-7-369 |
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author | Song, Yu Tao, Ling Shen, Xiangchun |
author_facet | Song, Yu Tao, Ling Shen, Xiangchun |
author_sort | Song, Yu |
collection | PubMed |
description | We present a different strategy for synthesizing the Au-γ-Fe(2)O(3) bifunctional nanoparticle by using a larger (50 nm) Au nanoparticle as the core surrounded by smaller (10 nm) γ-Fe(2)O(3) nanoparticles. The synthesis of the composite nanoparticles is quite facile based on a simple redox process whereby Fe(2+) is used to reduce Au(3+). The morphology and composition of the product is measured by transmission electron microscopy, X-ray powder diffraction and UV–vis spectroscopy. We demonstrate the utility of these as-prepared Au-γ-Fe(2)O(3) nanoparticles by showing they can be used to separate proteins in solution. For example, bovine serum is efficiently removed from an aqueous solution with the simple addition of the NPs and application of a small magnet. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis is performed to evaluate the fidelity and efficiency of the protein separation procedure. |
format | Online Article Text |
id | pubmed-3423036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-34230362012-08-20 Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof Song, Yu Tao, Ling Shen, Xiangchun Nanoscale Res Lett Nano Express We present a different strategy for synthesizing the Au-γ-Fe(2)O(3) bifunctional nanoparticle by using a larger (50 nm) Au nanoparticle as the core surrounded by smaller (10 nm) γ-Fe(2)O(3) nanoparticles. The synthesis of the composite nanoparticles is quite facile based on a simple redox process whereby Fe(2+) is used to reduce Au(3+). The morphology and composition of the product is measured by transmission electron microscopy, X-ray powder diffraction and UV–vis spectroscopy. We demonstrate the utility of these as-prepared Au-γ-Fe(2)O(3) nanoparticles by showing they can be used to separate proteins in solution. For example, bovine serum is efficiently removed from an aqueous solution with the simple addition of the NPs and application of a small magnet. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis is performed to evaluate the fidelity and efficiency of the protein separation procedure. Springer 2012-07-03 /pmc/articles/PMC3423036/ /pubmed/22759882 http://dx.doi.org/10.1186/1556-276X-7-369 Text en Copyright ©2012 Song et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Nano Express Song, Yu Tao, Ling Shen, Xiangchun Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof |
title | Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof |
title_full | Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof |
title_fullStr | Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof |
title_full_unstemmed | Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof |
title_short | Synthesis of new type of Au-magnetic nanocomposite and application for protein separation thereof |
title_sort | synthesis of new type of au-magnetic nanocomposite and application for protein separation thereof |
topic | Nano Express |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423036/ https://www.ncbi.nlm.nih.gov/pubmed/22759882 http://dx.doi.org/10.1186/1556-276X-7-369 |
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