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Evaluation of combined high-efficiency DNA extraction and real-time PCR for detection of Mycobacterium avium subsp. paratuberculosis in subclinically infected dairy cattle: comparison with faecal culture, milk real-time PCR and milk ELISA

BACKGROUND: Johne’s disease is caused by Mycobacterium avium subsp. paratuberculosis (Map) and it is one of the most important diseases in cattle worldwide. Several laboratory tests for Map detection are available; however, these are limited by inadequate sensitivity and specificity when used in sub...

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Detalles Bibliográficos
Autores principales:	Logar, Katarina, Kopinč, Rok, Bandelj, Petra, Starič, Jože, Lapanje, Aleš, Ocepek, Matjaž
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423054/ https://www.ncbi.nlm.nih.gov/pubmed/22551054 http://dx.doi.org/10.1186/1746-6148-8-49

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