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Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus

BACKGROUND: Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and iden...

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Autores principales: Pan, Qing, Liu, Aijing, Zhang, Faming, Ling, Yong, Ou, Changbo, Hou, Na, He, Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424113/
https://www.ncbi.nlm.nih.gov/pubmed/22748160
http://dx.doi.org/10.1186/1746-6148-8-104
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author Pan, Qing
Liu, Aijing
Zhang, Faming
Ling, Yong
Ou, Changbo
Hou, Na
He, Cheng
author_facet Pan, Qing
Liu, Aijing
Zhang, Faming
Ling, Yong
Ou, Changbo
Hou, Na
He, Cheng
author_sort Pan, Qing
collection PubMed
description BACKGROUND: Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and identify the pathogens that are responsible for the progressive pneumonia and establish an animal model for drug screening. RESULTS: 193 serum samples were collected from 8 intensive farms from 5 provinces in China and analysed in the current research. Our clinical survey showed that 65.2% to 100% of breeding broilers, breeding layers, broilers and laying hens were seropositive for ORT antibodies. From 8 intensive farms, six ORT isolates were identified by PCR and biochemical assays, and two H9N2 viruses were isolated. Newcastle Disease Virus (NDV) and Infectious BronchitisVirus (IBV) were excluded. Typical pneumonia and airsacculitis were observed both in broilers inoculated intraperitoneally with an ORT isolate alone and in those co-infected with ORT and H9N2 virus isolates. Specifically, the survival rate was 30%, 20%, 70%, 50% and 90% in birds inoculated with ORT+H9N2 virus, ORT followed by H9N2 virus, H9N2 virus followed by ORT, and ORT or H9N2 virus alone, respectively. CONCLUSIONS: The results of this study suggest that ORT infections of domestic poultry have been occurring frequently in China. ORT infection can induce higher economic losses and mortality if H9N2 AIV is also present. Although the isolation of ORT and H9N2 virus has been reported previously, there have been no reported co-infections of poultry with these two pathogens. This is the first report of co-infection of broilers with ORT and H9N2 virus, and this co-infection is probably associated with the outbreak of broiler airsacculitis in China, which has caused extensive economic losses.
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spelling pubmed-34241132012-08-22 Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus Pan, Qing Liu, Aijing Zhang, Faming Ling, Yong Ou, Changbo Hou, Na He, Cheng BMC Vet Res Research Article BACKGROUND: Since 2008, a progressive pneumonia has become prevalent in broilers and laying hens. This disease occurrs the first day after hatching and lasts more than 30 days, resulting in approximately 70% morbidity and 30% mortality in broilers. The objective of this study was to isolate and identify the pathogens that are responsible for the progressive pneumonia and establish an animal model for drug screening. RESULTS: 193 serum samples were collected from 8 intensive farms from 5 provinces in China and analysed in the current research. Our clinical survey showed that 65.2% to 100% of breeding broilers, breeding layers, broilers and laying hens were seropositive for ORT antibodies. From 8 intensive farms, six ORT isolates were identified by PCR and biochemical assays, and two H9N2 viruses were isolated. Newcastle Disease Virus (NDV) and Infectious BronchitisVirus (IBV) were excluded. Typical pneumonia and airsacculitis were observed both in broilers inoculated intraperitoneally with an ORT isolate alone and in those co-infected with ORT and H9N2 virus isolates. Specifically, the survival rate was 30%, 20%, 70%, 50% and 90% in birds inoculated with ORT+H9N2 virus, ORT followed by H9N2 virus, H9N2 virus followed by ORT, and ORT or H9N2 virus alone, respectively. CONCLUSIONS: The results of this study suggest that ORT infections of domestic poultry have been occurring frequently in China. ORT infection can induce higher economic losses and mortality if H9N2 AIV is also present. Although the isolation of ORT and H9N2 virus has been reported previously, there have been no reported co-infections of poultry with these two pathogens. This is the first report of co-infection of broilers with ORT and H9N2 virus, and this co-infection is probably associated with the outbreak of broiler airsacculitis in China, which has caused extensive economic losses. BioMed Central 2012-07-02 /pmc/articles/PMC3424113/ /pubmed/22748160 http://dx.doi.org/10.1186/1746-6148-8-104 Text en Copyright ©2012 Pan et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pan, Qing
Liu, Aijing
Zhang, Faming
Ling, Yong
Ou, Changbo
Hou, Na
He, Cheng
Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
title Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
title_full Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
title_fullStr Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
title_full_unstemmed Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
title_short Co-infection of broilers with Ornithobacterium rhinotracheale and H9N2 avian influenza virus
title_sort co-infection of broilers with ornithobacterium rhinotracheale and h9n2 avian influenza virus
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424113/
https://www.ncbi.nlm.nih.gov/pubmed/22748160
http://dx.doi.org/10.1186/1746-6148-8-104
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