Optimization and comparison of knockdown efficacy between polymerase II expressed shRNA and artificial miRNA targeting luciferase and Apolipoprotein B100

BACKGROUND: Controlling and limiting the expression of short hairpin RNA (shRNA) by using constitutive or tissue-specific polymerase II (pol II) expression can be a promising strategy to avoid RNAi toxicity. However, to date detailed studies on requirements for effective pol II shRNA expression and...

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Detalles Bibliográficos
Autores principales: Maczuga, Piotr, Koornneef, Annemart, Borel, Florie, Petry, Harald, van Deventer, Sander, Ritsema, Tita, Konstantinova, Pavlina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424168/
https://www.ncbi.nlm.nih.gov/pubmed/22827812
http://dx.doi.org/10.1186/1472-6750-12-42

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