Targeted gene knockout by direct delivery of ZFN proteins

Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standar...

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Detalles Bibliográficos
Autores principales: Gaj, Thomas, Guo, Jing, Kato, Yoshio, Sirk, Shannon J., Barbas, Carlos F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424280/
https://www.ncbi.nlm.nih.gov/pubmed/22751204
http://dx.doi.org/10.1038/nmeth.2030
Descripción
Sumario:Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standard ZFN architecture and show that direct delivery of ZFNs as proteins leads to efficient endogenous gene disruption in a variety of mammalian cell types with minimal off-target effects.

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