Targeted gene knockout by direct delivery of ZFN proteins

Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standar...

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Detalles Bibliográficos
Autores principales: Gaj, Thomas, Guo, Jing, Kato, Yoshio, Sirk, Shannon J., Barbas, Carlos F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424280/
https://www.ncbi.nlm.nih.gov/pubmed/22751204
http://dx.doi.org/10.1038/nmeth.2030
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author Gaj, Thomas
Guo, Jing
Kato, Yoshio
Sirk, Shannon J.
Barbas, Carlos F.
author_facet Gaj, Thomas
Guo, Jing
Kato, Yoshio
Sirk, Shannon J.
Barbas, Carlos F.
author_sort Gaj, Thomas
collection PubMed
description Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standard ZFN architecture and show that direct delivery of ZFNs as proteins leads to efficient endogenous gene disruption in a variety of mammalian cell types with minimal off-target effects.
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spelling pubmed-34242802013-02-01 Targeted gene knockout by direct delivery of ZFN proteins Gaj, Thomas Guo, Jing Kato, Yoshio Sirk, Shannon J. Barbas, Carlos F. Nat Methods Article Zinc-finger nucleases (ZFNs) are versatile reagents that have redefined genome engineering. Realizing the full potential of this technology requires the development of safe and effective methods for delivering ZFNs into cells. We demonstrate the intrinsic cell-penetrating capabilities of the standard ZFN architecture and show that direct delivery of ZFNs as proteins leads to efficient endogenous gene disruption in a variety of mammalian cell types with minimal off-target effects. 2012-07-01 /pmc/articles/PMC3424280/ /pubmed/22751204 http://dx.doi.org/10.1038/nmeth.2030 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Gaj, Thomas
Guo, Jing
Kato, Yoshio
Sirk, Shannon J.
Barbas, Carlos F.
Targeted gene knockout by direct delivery of ZFN proteins
title Targeted gene knockout by direct delivery of ZFN proteins
title_full Targeted gene knockout by direct delivery of ZFN proteins
title_fullStr Targeted gene knockout by direct delivery of ZFN proteins
title_full_unstemmed Targeted gene knockout by direct delivery of ZFN proteins
title_short Targeted gene knockout by direct delivery of ZFN proteins
title_sort targeted gene knockout by direct delivery of zfn proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424280/
https://www.ncbi.nlm.nih.gov/pubmed/22751204
http://dx.doi.org/10.1038/nmeth.2030
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AT sirkshannonj targetedgeneknockoutbydirectdeliveryofzfnproteins
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