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Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence

Tuberculosis, caused by Mycobacterium tuberculosis, remains a leading infectious disease taking one human life every 15 s globally. The two well-characterized strains H(37)Rv and H(37)Ra, derived from the same parental strain M. tuberculosis H(37), show dramatically different pathogenic phenotypes....

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Autores principales: Kohli, Sakshi, Singh, Yadvir, Sharma, Khushbu, Mittal, Aditya, Ehtesham, Nasreen Z., Hasnain, Seyed E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424577/
https://www.ncbi.nlm.nih.gov/pubmed/22618876
http://dx.doi.org/10.1093/nar/gks465
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author Kohli, Sakshi
Singh, Yadvir
Sharma, Khushbu
Mittal, Aditya
Ehtesham, Nasreen Z.
Hasnain, Seyed E.
author_facet Kohli, Sakshi
Singh, Yadvir
Sharma, Khushbu
Mittal, Aditya
Ehtesham, Nasreen Z.
Hasnain, Seyed E.
author_sort Kohli, Sakshi
collection PubMed
description Tuberculosis, caused by Mycobacterium tuberculosis, remains a leading infectious disease taking one human life every 15 s globally. The two well-characterized strains H(37)Rv and H(37)Ra, derived from the same parental strain M. tuberculosis H(37), show dramatically different pathogenic phenotypes. PE/PPE gene family, comprising of 176 open reading frames and present exclusively in genus Mycobacterium, accounts for ∼10% of the M. tuberculosis genome. Our comprehensive in silico analyses of PE/PPE family of H(37)Ra and virulent H(37)Rv strains revealed genetic differences between these strains in terms of several single nucleotide variations and InDels and these manifested in changes in physico-chemical properties, phosphorylation sites, and protein: protein interacting domains of the corresponding proteomes. Similar comparisons using the 13 sigma factor genes, 36 members of the mammalian cell entry family, 13 mycobacterial membrane protein large family members and 11 two-component signal transduction systems along with 5 orphaned response regulators and 2 orphaned sensor kinases failed to reveal very significant difference between H(37)Rv and H(37)Ra, reinforcing the importance of PE/PPE genes. Many of these changes between H(37)Rv and H(37)Ra can be correlated to differences in pathogenesis and virulence of the two strains.
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spelling pubmed-34245772012-08-22 Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence Kohli, Sakshi Singh, Yadvir Sharma, Khushbu Mittal, Aditya Ehtesham, Nasreen Z. Hasnain, Seyed E. Nucleic Acids Res Computational Biology Tuberculosis, caused by Mycobacterium tuberculosis, remains a leading infectious disease taking one human life every 15 s globally. The two well-characterized strains H(37)Rv and H(37)Ra, derived from the same parental strain M. tuberculosis H(37), show dramatically different pathogenic phenotypes. PE/PPE gene family, comprising of 176 open reading frames and present exclusively in genus Mycobacterium, accounts for ∼10% of the M. tuberculosis genome. Our comprehensive in silico analyses of PE/PPE family of H(37)Ra and virulent H(37)Rv strains revealed genetic differences between these strains in terms of several single nucleotide variations and InDels and these manifested in changes in physico-chemical properties, phosphorylation sites, and protein: protein interacting domains of the corresponding proteomes. Similar comparisons using the 13 sigma factor genes, 36 members of the mammalian cell entry family, 13 mycobacterial membrane protein large family members and 11 two-component signal transduction systems along with 5 orphaned response regulators and 2 orphaned sensor kinases failed to reveal very significant difference between H(37)Rv and H(37)Ra, reinforcing the importance of PE/PPE genes. Many of these changes between H(37)Rv and H(37)Ra can be correlated to differences in pathogenesis and virulence of the two strains. Oxford University Press 2012-08 2012-05-22 /pmc/articles/PMC3424577/ /pubmed/22618876 http://dx.doi.org/10.1093/nar/gks465 Text en © The Author(s) 2012. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Computational Biology
Kohli, Sakshi
Singh, Yadvir
Sharma, Khushbu
Mittal, Aditya
Ehtesham, Nasreen Z.
Hasnain, Seyed E.
Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence
title Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence
title_full Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence
title_fullStr Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence
title_full_unstemmed Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence
title_short Comparative genomic and proteomic analyses of PE/PPE multigene family of Mycobacterium tuberculosis H(37)Rv and H(37)Ra reveal novel and interesting differences with implications in virulence
title_sort comparative genomic and proteomic analyses of pe/ppe multigene family of mycobacterium tuberculosis h(37)rv and h(37)ra reveal novel and interesting differences with implications in virulence
topic Computational Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3424577/
https://www.ncbi.nlm.nih.gov/pubmed/22618876
http://dx.doi.org/10.1093/nar/gks465
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