A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media

BACKGROUND: Haemophilus influenzae requires heme for aerobic growth and possesses multiple mechanisms to obtain this essential nutrient. METHODS: An insertional mutation in tonB was constructed and the impact of the mutation on virulence and fitness in a chinchilla model of otitis media was determin...

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Autores principales: Morton, Daniel J, Hempel, Randy J, Seale, Thomas W, Whitby, Paul W, Stull, Terrence L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425130/
https://www.ncbi.nlm.nih.gov/pubmed/22731867
http://dx.doi.org/10.1186/1756-0500-5-327
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author Morton, Daniel J
Hempel, Randy J
Seale, Thomas W
Whitby, Paul W
Stull, Terrence L
author_facet Morton, Daniel J
Hempel, Randy J
Seale, Thomas W
Whitby, Paul W
Stull, Terrence L
author_sort Morton, Daniel J
collection PubMed
description BACKGROUND: Haemophilus influenzae requires heme for aerobic growth and possesses multiple mechanisms to obtain this essential nutrient. METHODS: An insertional mutation in tonB was constructed and the impact of the mutation on virulence and fitness in a chinchilla model of otitis media was determined. The tonB insertion mutant strain was significantly impacted in both virulence and fitness as compared to the wildtype strain in this model. CONCLUSIONS: The tonB gene of H. influenzae is required for the establishment and maintenance of middle ear infection in this chinchilla model of bacterial disease.
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spelling pubmed-34251302012-08-23 A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media Morton, Daniel J Hempel, Randy J Seale, Thomas W Whitby, Paul W Stull, Terrence L BMC Res Notes Research Article BACKGROUND: Haemophilus influenzae requires heme for aerobic growth and possesses multiple mechanisms to obtain this essential nutrient. METHODS: An insertional mutation in tonB was constructed and the impact of the mutation on virulence and fitness in a chinchilla model of otitis media was determined. The tonB insertion mutant strain was significantly impacted in both virulence and fitness as compared to the wildtype strain in this model. CONCLUSIONS: The tonB gene of H. influenzae is required for the establishment and maintenance of middle ear infection in this chinchilla model of bacterial disease. BioMed Central 2012-06-25 /pmc/articles/PMC3425130/ /pubmed/22731867 http://dx.doi.org/10.1186/1756-0500-5-327 Text en Copyright ©2012 Morton et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Morton, Daniel J
Hempel, Randy J
Seale, Thomas W
Whitby, Paul W
Stull, Terrence L
A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media
title A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media
title_full A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media
title_fullStr A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media
title_full_unstemmed A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media
title_short A functional tonB gene is required for both virulence and competitive fitness in a chinchilla model of Haemophilus influenzae otitis media
title_sort functional tonb gene is required for both virulence and competitive fitness in a chinchilla model of haemophilus influenzae otitis media
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425130/
https://www.ncbi.nlm.nih.gov/pubmed/22731867
http://dx.doi.org/10.1186/1756-0500-5-327
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