Cargando…

Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever

Protection of cattle from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever (MCF) has been described previously, using an attenuated virus vaccine in an unlicensed adjuvant. The vaccine was hypothesised to induce a protective barrier of virus-neutralising antibody in the oro-nasal...

Descripción completa

Detalles Bibliográficos
Autores principales: Russell, George C, Benavides, Julio, Grant, Dawn, Todd, Helen, Deane, David, Percival, Ann, Thomson, Jackie, Connelly, Maira, Haig, David M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425131/
https://www.ncbi.nlm.nih.gov/pubmed/22686373
http://dx.doi.org/10.1186/1297-9716-43-51
_version_ 1782241332655292416
author Russell, George C
Benavides, Julio
Grant, Dawn
Todd, Helen
Deane, David
Percival, Ann
Thomson, Jackie
Connelly, Maira
Haig, David M
author_facet Russell, George C
Benavides, Julio
Grant, Dawn
Todd, Helen
Deane, David
Percival, Ann
Thomson, Jackie
Connelly, Maira
Haig, David M
author_sort Russell, George C
collection PubMed
description Protection of cattle from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever (MCF) has been described previously, using an attenuated virus vaccine in an unlicensed adjuvant. The vaccine was hypothesised to induce a protective barrier of virus-neutralising antibody in the oro-nasal region, supported by the observation of high titre neutralising antibodies in nasal secretions of protected animals. Here we describe further analysis of this vaccine strategy, studying the effectiveness of the vaccine formulated with a licensed adjuvant; the duration of immunity induced; and the virus-specific antibody responses in plasma and nasal secretions. The results presented here show that the attenuated AlHV-1 vaccine in a licensed adjuvant protected cattle from fatal intranasal challenge with pathogenic AlHV-1 at three or six months. In addition, animals protected from MCF had significantly higher initial anti-viral antibody titres than animals that succumbed to disease; and these antibody titres remained relatively stable after challenge, while titres in vaccinated animals with MCF increased significantly prior to the onset of clinical disease. These data support the view that a mucosal barrier of neutralising antibody blocks infection of vaccinated animals and suggests that the magnitude of the initial response may correlate with long-term protection. Interestingly, the high titre virus-neutralising antibody responses seen in animals that succumbed to MCF after vaccination were not protective.
format Online
Article
Text
id pubmed-3425131
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-34251312012-08-23 Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever Russell, George C Benavides, Julio Grant, Dawn Todd, Helen Deane, David Percival, Ann Thomson, Jackie Connelly, Maira Haig, David M Vet Res Research Protection of cattle from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever (MCF) has been described previously, using an attenuated virus vaccine in an unlicensed adjuvant. The vaccine was hypothesised to induce a protective barrier of virus-neutralising antibody in the oro-nasal region, supported by the observation of high titre neutralising antibodies in nasal secretions of protected animals. Here we describe further analysis of this vaccine strategy, studying the effectiveness of the vaccine formulated with a licensed adjuvant; the duration of immunity induced; and the virus-specific antibody responses in plasma and nasal secretions. The results presented here show that the attenuated AlHV-1 vaccine in a licensed adjuvant protected cattle from fatal intranasal challenge with pathogenic AlHV-1 at three or six months. In addition, animals protected from MCF had significantly higher initial anti-viral antibody titres than animals that succumbed to disease; and these antibody titres remained relatively stable after challenge, while titres in vaccinated animals with MCF increased significantly prior to the onset of clinical disease. These data support the view that a mucosal barrier of neutralising antibody blocks infection of vaccinated animals and suggests that the magnitude of the initial response may correlate with long-term protection. Interestingly, the high titre virus-neutralising antibody responses seen in animals that succumbed to MCF after vaccination were not protective. BioMed Central 2012 2012-06-11 /pmc/articles/PMC3425131/ /pubmed/22686373 http://dx.doi.org/10.1186/1297-9716-43-51 Text en Copyright ©2012 Russell et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Russell, George C
Benavides, Julio
Grant, Dawn
Todd, Helen
Deane, David
Percival, Ann
Thomson, Jackie
Connelly, Maira
Haig, David M
Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
title Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
title_full Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
title_fullStr Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
title_full_unstemmed Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
title_short Duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
title_sort duration of protective immunity and antibody responses in cattle immunised against alcelaphine herpesvirus-1-induced malignant catarrhal fever
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425131/
https://www.ncbi.nlm.nih.gov/pubmed/22686373
http://dx.doi.org/10.1186/1297-9716-43-51
work_keys_str_mv AT russellgeorgec durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT benavidesjulio durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT grantdawn durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT toddhelen durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT deanedavid durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT percivalann durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT thomsonjackie durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT connellymaira durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever
AT haigdavidm durationofprotectiveimmunityandantibodyresponsesincattleimmunisedagainstalcelaphineherpesvirus1inducedmalignantcatarrhalfever