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Rhamnose-Inducible Gene Expression in Listeria monocytogenes
Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) o...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425472/ https://www.ncbi.nlm.nih.gov/pubmed/22927968 http://dx.doi.org/10.1371/journal.pone.0043444 |
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author | Fieseler, Lars Schmitter, Sibylle Teiserskas, Justinas Loessner, Martin J. |
author_facet | Fieseler, Lars Schmitter, Sibylle Teiserskas, Justinas Loessner, Martin J. |
author_sort | Fieseler, Lars |
collection | PubMed |
description | Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in L. monocytogenes. Transcription initiation from P(rha) is dose dependent, and a concentration as low as 100 µM rhamnose was found sufficient for induction. Moreover, P(rha) is subject to glucose catabolite repression, which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that P(rha) is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible interference by catabolite repression. The P(rha) promoter provides a novel and useful tool for triggering gene expression in extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter. |
format | Online Article Text |
id | pubmed-3425472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34254722012-08-27 Rhamnose-Inducible Gene Expression in Listeria monocytogenes Fieseler, Lars Schmitter, Sibylle Teiserskas, Justinas Loessner, Martin J. PLoS One Research Article Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in L. monocytogenes. Transcription initiation from P(rha) is dose dependent, and a concentration as low as 100 µM rhamnose was found sufficient for induction. Moreover, P(rha) is subject to glucose catabolite repression, which provides additional options for strict control of expression. Infection of human THP1 macrophages revealed that P(rha) is repressed in intracellular L. monocytogenes, which is explained by the absence of rhamnose in the cytosol and possible interference by catabolite repression. The P(rha) promoter provides a novel and useful tool for triggering gene expression in extracellular L. monocytogenes, whereas intracellular conditions prevent transcription from this promoter. Public Library of Science 2012-08-22 /pmc/articles/PMC3425472/ /pubmed/22927968 http://dx.doi.org/10.1371/journal.pone.0043444 Text en © 2012 Fieseler et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Fieseler, Lars Schmitter, Sibylle Teiserskas, Justinas Loessner, Martin J. Rhamnose-Inducible Gene Expression in Listeria monocytogenes |
title | Rhamnose-Inducible Gene Expression in Listeria monocytogenes
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title_full | Rhamnose-Inducible Gene Expression in Listeria monocytogenes
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title_fullStr | Rhamnose-Inducible Gene Expression in Listeria monocytogenes
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title_full_unstemmed | Rhamnose-Inducible Gene Expression in Listeria monocytogenes
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title_short | Rhamnose-Inducible Gene Expression in Listeria monocytogenes
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title_sort | rhamnose-inducible gene expression in listeria monocytogenes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425472/ https://www.ncbi.nlm.nih.gov/pubmed/22927968 http://dx.doi.org/10.1371/journal.pone.0043444 |
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