Cargando…

Quantitative Analysis of α-Synuclein Solubility in Living Cells Using Split GFP Complementation

Presently incurable, Parkinson's disease (PD) is the most common neurodegenerative movement disorder and affects 1% of the population over 60 years of age. The hallmarks of PD pathogenesis are the loss of dopaminergic neurons in the substantia nigra pars compacta, and the occurrence of proteina...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kothawala, Ahmed, Kilpatrick, Kiri, Novoa, Jose Andres, Segatori, Laura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425482/ https://www.ncbi.nlm.nih.gov/pubmed/22927976 http://dx.doi.org/10.1371/journal.pone.0043505

Ejemplares similares

Genetic and Chemical Activation of TFEB Mediates Clearance of Aggregated α-Synuclein
por: Kilpatrick, Kiri, et al.
Publicado: (2015)

A split-GFP tool reveals differences in the sub-mitochondrial distribution of wt and mutant alpha-synuclein
por: Vicario, Mattia, et al.
Publicado: (2019)

splitGFP Technology Reveals Dose-Dependent ER-Mitochondria Interface Modulation by α-Synuclein A53T and A30P Mutants
por: Calì, Tito, et al.
Publicado: (2019)

Snca-GFP Knock-In Mice Allows Tracking the Endogenous α-Synuclein in Action
por: Er, Safak
Publicado: (2021)

GFP-complementation assay to detect functional CPP and protein delivery into living cells
por: Milech, Nadia, et al.
Publicado: (2015)

Ebola and Marburg virus VP35 coiled-coil validated as antiviral target by tripartite split-GFP complementation
por: Zinzula, Luca, et al.
Publicado: (2022)

Longitudinal live imaging of retinal α-synuclein::GFP deposits in a transgenic mouse model of Parkinson’s Disease/Dementia with Lewy Bodies
por: Price, Diana L., et al.
Publicado: (2016)

Resistance to MPTP-Neurotoxicity in α-Synuclein Knockout Mice Is Complemented by Human α-Synuclein and Associated with Increased β-Synuclein and Akt Activation
por: Thomas, Bobby, et al.
Publicado: (2011)

Fluorescent Labeling of Antibody Fragments Using Split GFP
por: Ferrara, Fortunato, et al.
Publicado: (2011)

Correction: Resistance to MPTP-Neurotoxicity in α-Synuclein Knockout Mice Is Complemented by Human α-Synuclein and Associated with Increased β-Synuclein and Akt Activation
por: Thomas, Bobby, et al.
Publicado: (2012)

Illuminating the Sites of Enterovirus Replication in Living Cells by Using a Split-GFP-Tagged Viral Protein
por: van der Schaar, H. M., et al.
Publicado: (2016)

Tissue-Specific Split sfGFP System for Streamlined Expression of GFP Tagged Proteins in the Caenorhabditis elegans Germline
por: Hefel, Adam, et al.
Publicado: (2019)

Scaling production of GFP1-10 detector protein in E. coli for secretion screening by split GFP assay
por: Müller, Carolin, et al.
Publicado: (2021)

Specific cell surface labeling of GPCRs using split GFP
por: Jiang, Wen-Xue, et al.
Publicado: (2016)

Interaction of Haloarchaeal Gas Vesicle Proteins Determined by Split-GFP
por: Winter, Kerstin, et al.
Publicado: (2018)

Dopamine induces soluble α-synuclein oligomers and nigrostriatal degeneration
por: Mor, Danielle E., et al.
Publicado: (2017)

Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins in Caenorhabditis elegans
por: Goudeau, Jérôme, et al.
Publicado: (2021)

Quantitative Characterization of α-Synuclein Aggregation in Living Cells through Automated Microfluidics Feedback Control
por: Perrino, Giansimone, et al.
Publicado: (2019)

Intracellular soluble α‐synuclein oligomers reduce pyramidal cell excitability
por: Kaufmann, Timothy J., et al.
Publicado: (2016)

Small soluble α-synuclein aggregates are the toxic species in Parkinson’s disease
por: Emin, Derya, et al.
Publicado: (2022)

Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses
por: Ferrari, Elena, et al.
Publicado: (2023)

Protein stabilization with retained function of monellin using a split GFP system
por: Weiffert, Tanja, et al.
Publicado: (2018)

The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis
por: Lenz, Patrick, et al.
Publicado: (2021)

Split-GFP Reassembly Assay: Strengths and Caveats from a Multiparametric Analysis
por: Bignon, Christophe, et al.
Publicado: (2022)

Complementation Assay Using Fusion of Split-GFP and TurboID (CsFiND) Enables Simultaneous Visualization and Proximity Labeling of Organelle Contact Sites in Yeast
por: Fujimoto, Shintaro, et al.
Publicado: (2023)

Opposed Effects of Dityrosine Formation in Soluble and Aggregated α-Synuclein on Fibril Growth
por: Wördehoff, Michael M., et al.
Publicado: (2017)

A toxic subset of soluble α-synuclein species in dementia with Lewy body
por: Castillo-Carranza, Diana L
Publicado: (2020)

A New Method for Quantitative Immunoblotting of Endogenous α-Synuclein
por: Newman, Andrew J., et al.
Publicado: (2013)

A New Protein-Protein Interaction Sensor Based on Tripartite Split-GFP Association
por: Cabantous, Stéphanie, et al.
Publicado: (2013)

A Split-GFP Gateway Cloning System for Topology Analyses of Membrane Proteins in Plants
por: Xie, Wenjun, et al.
Publicado: (2017)

Chromophore pre-maturation for improved speed and sensitivity of split-GFP monitoring of protein secretion
por: Lundqvist, Magnus, et al.
Publicado: (2019)

Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells
por: Tashiro, Shinya, et al.
Publicado: (2020)

A BIOCHEMICAL STUDY OF THE PHENOMENA KNOWN AS COMPLEMENT-SPLITTING : FIRST PAPER: SPLITTING OF THE COMPLEMENT ASSOCIATED WITH GLOBULIN PRECIPITATION.
por: Bronfenbrenner, Jacob, et al.
Publicado: (1912)

Formation of Toxic Oligomeric α-Synuclein Species in Living Cells
por: Outeiro, Tiago Fleming, et al.
Publicado: (2008)

Diffusion of a soluble protein, photoactivatable GFP, through a sensory cilium
por: Calvert, Peter D., et al.
Publicado: (2010)

Split GFP technologies to structurally characterize and quantify functional biomolecular interactions of FTD-related proteins
por: Foglieni, Chiara, et al.
Publicado: (2017)

Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system
por: Kakimoto, Yuriko, et al.
Publicado: (2018)

In Vivo Protein–Protein Binding Competition Assay Based on Split-GFP Reassembly: Proof of Concept
por: Bignon, Christophe, et al.
Publicado: (2023)

A Split-Luciferase Reporter Recognizing GFP and mCherry Tags to Facilitate Studies of Protein–Protein Interactions
por: Moustaqil, Mehdi, et al.
Publicado: (2017)

A split luciferase-based probe for quantitative proximal determination of Gα(q) signalling in live cells
por: Littmann, Timo, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_g6il85g8nsi93vgptt4bl0cal0