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A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability

Malignant melanoma is an aggressive form of skin cancer with poor prognosis. Despite improvements in awareness and prevention of this disease, its incidence is rapidly increasing. MicroRNAs (miRNAs) are a class of small RNA molecules that regulate cellular processes by repressing messenger RNAs (mRN...

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Autores principales: Poell, Jos B., van Haastert, Rick J., de Gunst, Thijs, Schultz, Iman J., Gommans, Willemijn M., Verheul, Mark, Cerisoli, Francesco, van Noort, Paula I., Prevost, Gregoire P., Schaapveld, Roel Q. J., Cuppen, Edwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425484/
https://www.ncbi.nlm.nih.gov/pubmed/22927992
http://dx.doi.org/10.1371/journal.pone.0043569
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author Poell, Jos B.
van Haastert, Rick J.
de Gunst, Thijs
Schultz, Iman J.
Gommans, Willemijn M.
Verheul, Mark
Cerisoli, Francesco
van Noort, Paula I.
Prevost, Gregoire P.
Schaapveld, Roel Q. J.
Cuppen, Edwin
author_facet Poell, Jos B.
van Haastert, Rick J.
de Gunst, Thijs
Schultz, Iman J.
Gommans, Willemijn M.
Verheul, Mark
Cerisoli, Francesco
van Noort, Paula I.
Prevost, Gregoire P.
Schaapveld, Roel Q. J.
Cuppen, Edwin
author_sort Poell, Jos B.
collection PubMed
description Malignant melanoma is an aggressive form of skin cancer with poor prognosis. Despite improvements in awareness and prevention of this disease, its incidence is rapidly increasing. MicroRNAs (miRNAs) are a class of small RNA molecules that regulate cellular processes by repressing messenger RNAs (mRNAs) with partially complementary target sites. Several miRNAs have already been shown to attenuate cancer phenotypes, by limiting proliferation, invasiveness, tumor angiogenesis, and stemness. Here, we employed a genome-scale lentiviral human miRNA expression library to systematically survey which miRNAs are able to decrease A375 melanoma cell viability. We highlight the strongest inhibitors of melanoma cell proliferation, including the miR-15/16, miR-141/200a and miR-96/182 families of miRNAs and miR-203. Ectopic expression of these miRNAs resulted in long-term inhibition of melanoma cell expansion, both in vitro and in vivo. We show specifically miR-16, miR-497, miR-96 and miR-182 are efficient effectors when introduced as synthetic miRNAs in several melanoma cell lines. Our study provides a comprehensive interrogation of miRNAs that interfere with melanoma cell proliferation and viability, and offers a selection of miRNAs that are especially promising candidates for application in melanoma therapy.
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spelling pubmed-34254842012-08-27 A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability Poell, Jos B. van Haastert, Rick J. de Gunst, Thijs Schultz, Iman J. Gommans, Willemijn M. Verheul, Mark Cerisoli, Francesco van Noort, Paula I. Prevost, Gregoire P. Schaapveld, Roel Q. J. Cuppen, Edwin PLoS One Research Article Malignant melanoma is an aggressive form of skin cancer with poor prognosis. Despite improvements in awareness and prevention of this disease, its incidence is rapidly increasing. MicroRNAs (miRNAs) are a class of small RNA molecules that regulate cellular processes by repressing messenger RNAs (mRNAs) with partially complementary target sites. Several miRNAs have already been shown to attenuate cancer phenotypes, by limiting proliferation, invasiveness, tumor angiogenesis, and stemness. Here, we employed a genome-scale lentiviral human miRNA expression library to systematically survey which miRNAs are able to decrease A375 melanoma cell viability. We highlight the strongest inhibitors of melanoma cell proliferation, including the miR-15/16, miR-141/200a and miR-96/182 families of miRNAs and miR-203. Ectopic expression of these miRNAs resulted in long-term inhibition of melanoma cell expansion, both in vitro and in vivo. We show specifically miR-16, miR-497, miR-96 and miR-182 are efficient effectors when introduced as synthetic miRNAs in several melanoma cell lines. Our study provides a comprehensive interrogation of miRNAs that interfere with melanoma cell proliferation and viability, and offers a selection of miRNAs that are especially promising candidates for application in melanoma therapy. Public Library of Science 2012-08-22 /pmc/articles/PMC3425484/ /pubmed/22927992 http://dx.doi.org/10.1371/journal.pone.0043569 Text en © 2012 Poell et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Poell, Jos B.
van Haastert, Rick J.
de Gunst, Thijs
Schultz, Iman J.
Gommans, Willemijn M.
Verheul, Mark
Cerisoli, Francesco
van Noort, Paula I.
Prevost, Gregoire P.
Schaapveld, Roel Q. J.
Cuppen, Edwin
A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability
title A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability
title_full A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability
title_fullStr A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability
title_full_unstemmed A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability
title_short A Functional Screen Identifies Specific MicroRNAs Capable of Inhibiting Human Melanoma Cell Viability
title_sort functional screen identifies specific micrornas capable of inhibiting human melanoma cell viability
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425484/
https://www.ncbi.nlm.nih.gov/pubmed/22927992
http://dx.doi.org/10.1371/journal.pone.0043569
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