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Pseudogenes as Weaknesses of ACTB (Actb) and GAPDH (Gapdh) Used as Reference Genes in Reverse Transcription and Polymerase Chain Reactions

The genes encoding β-actin (ACTB in human or Actb in mouse) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH in human or Gapdh in mouse) are the two most commonly used references for sample normalization in determination of the mRNA level of interested genes by reverse transcription (RT) and ensu...

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Detalles Bibliográficos
Autores principales: Sun, Yuan, Li, Yan, Luo, Dianzhong, Liao, D. Joshua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425558/
https://www.ncbi.nlm.nih.gov/pubmed/22927912
http://dx.doi.org/10.1371/journal.pone.0041659

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