Cargando…

Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate

Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained fro...

Descripción completa

Detalles Bibliográficos
Autores principales: Steele, Ann, Boucek, Robert J., Jacobs, Jeffrey Phillip, Steele, Peter, Asante-Korang, Alfred, Chamizo, Wilfredo, Steele, Jasmine, Chai, Paul J., Quintessenza, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425869/
https://www.ncbi.nlm.nih.gov/pubmed/22936950
http://dx.doi.org/10.1155/2012/452102
_version_ 1782241429341339648
author Steele, Ann
Boucek, Robert J.
Jacobs, Jeffrey Phillip
Steele, Peter
Asante-Korang, Alfred
Chamizo, Wilfredo
Steele, Jasmine
Chai, Paul J.
Quintessenza, James A.
author_facet Steele, Ann
Boucek, Robert J.
Jacobs, Jeffrey Phillip
Steele, Peter
Asante-Korang, Alfred
Chamizo, Wilfredo
Steele, Jasmine
Chai, Paul J.
Quintessenza, James A.
author_sort Steele, Ann
collection PubMed
description Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n = 25; hAA-CHF). Minced tissues were suspended in complete (serum-containing) DMEM. Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit). Characterization of hc-kit(positive) cells included immunohistochemical screening with a panel of monoclonal antibodies. Results. Cells, including phase-bright cells identified as hc-kit(positive), spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC) after Day 7. When cocultured with tissue, emigrated hc-kit(positive) cells proliferated, first as loosely attached clones and later as multicellular clusters. At Day 21~5% of cells were hc-kit(positive). Between Days 14 and 28 hc-kit(positive) cells exhibited mesodermal commitment (GATA-4(positive) and NKX2.5(positive)); then after Day 28 cardiac lineages (flk-1(positive), smooth muscle actin(positive), troponin-I(positive), and myosin light chain(positive)). Conclusions. C-kit(positive) hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF. SEC is a novel culture method for derivation of migratory hc-kit(positive) cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro.
format Online
Article
Text
id pubmed-3425869
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-34258692012-08-30 Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate Steele, Ann Boucek, Robert J. Jacobs, Jeffrey Phillip Steele, Peter Asante-Korang, Alfred Chamizo, Wilfredo Steele, Jasmine Chai, Paul J. Quintessenza, James A. Stem Cells Int Clinical Study Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients (n = 25; hAA-CHF). Minced tissues were suspended in complete (serum-containing) DMEM. Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit). Characterization of hc-kit(positive) cells included immunohistochemical screening with a panel of monoclonal antibodies. Results. Cells, including phase-bright cells identified as hc-kit(positive), spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC) after Day 7. When cocultured with tissue, emigrated hc-kit(positive) cells proliferated, first as loosely attached clones and later as multicellular clusters. At Day 21~5% of cells were hc-kit(positive). Between Days 14 and 28 hc-kit(positive) cells exhibited mesodermal commitment (GATA-4(positive) and NKX2.5(positive)); then after Day 28 cardiac lineages (flk-1(positive), smooth muscle actin(positive), troponin-I(positive), and myosin light chain(positive)). Conclusions. C-kit(positive) hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF. SEC is a novel culture method for derivation of migratory hc-kit(positive) cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro. Hindawi Publishing Corporation 2012 2012-08-14 /pmc/articles/PMC3425869/ /pubmed/22936950 http://dx.doi.org/10.1155/2012/452102 Text en Copyright © 2012 Ann Steele et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Clinical Study
Steele, Ann
Boucek, Robert J.
Jacobs, Jeffrey Phillip
Steele, Peter
Asante-Korang, Alfred
Chamizo, Wilfredo
Steele, Jasmine
Chai, Paul J.
Quintessenza, James A.
Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
title Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
title_full Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
title_fullStr Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
title_full_unstemmed Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
title_short Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate
title_sort heart cells with regenerative potential from pediatric patients with end stage heart failure: a translatable method to enrich and propagate
topic Clinical Study
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3425869/
https://www.ncbi.nlm.nih.gov/pubmed/22936950
http://dx.doi.org/10.1155/2012/452102
work_keys_str_mv AT steeleann heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT boucekrobertj heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT jacobsjeffreyphillip heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT steelepeter heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT asantekorangalfred heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT chamizowilfredo heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT steelejasmine heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT chaipaulj heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate
AT quintessenzajamesa heartcellswithregenerativepotentialfrompediatricpatientswithendstageheartfailureatranslatablemethodtoenrichandpropagate