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Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection

The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to co...

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Autores principales: Martins, Vicente P., Pinheiro, Carina S., Figueiredo, Barbara C. P., Assis, Natan R. G., Morais, Suellen B., Caliari, Marcelo V., Azevedo, Vasco, Castro-Borges, William, Wilson, R. Alan, Oliveira, Sergio C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426240/
https://www.ncbi.nlm.nih.gov/pubmed/22927873
http://dx.doi.org/10.1155/2012/962538
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author Martins, Vicente P.
Pinheiro, Carina S.
Figueiredo, Barbara C. P.
Assis, Natan R. G.
Morais, Suellen B.
Caliari, Marcelo V.
Azevedo, Vasco
Castro-Borges, William
Wilson, R. Alan
Oliveira, Sergio C.
author_facet Martins, Vicente P.
Pinheiro, Carina S.
Figueiredo, Barbara C. P.
Assis, Natan R. G.
Morais, Suellen B.
Caliari, Marcelo V.
Azevedo, Vasco
Castro-Borges, William
Wilson, R. Alan
Oliveira, Sergio C.
author_sort Martins, Vicente P.
collection PubMed
description The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate.
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spelling pubmed-34262402012-08-27 Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection Martins, Vicente P. Pinheiro, Carina S. Figueiredo, Barbara C. P. Assis, Natan R. G. Morais, Suellen B. Caliari, Marcelo V. Azevedo, Vasco Castro-Borges, William Wilson, R. Alan Oliveira, Sergio C. Clin Dev Immunol Research Article The flatworm Schistosoma mansoni is a blood fluke parasite that causes schistosomiasis, a debilitating disease that occurs throughout the developing world. Current schistosomiasis control strategies are mainly based on chemotherapy, but many researchers believe that the best long-term strategy to control schistosomiasis is through immunization with an antischistosomiasis vaccine combined with drug treatment. In the search for potential vaccine candidates, numerous tegument antigens have been assessed. As the major interface between parasite and mammalian host, the tegument plays crucial roles in the establishment and further course of schistosomiasis. Herein, we evaluated the potential of a GPI fraction, containing representative molecules located on the outer surface of adult worms, as vaccine candidate. Immunization of mice with GPI-anchored proteins induced a mixed Th1/Th2 type of immune response with production of IFN-γ and TNF-α, and low levels of IL-5 into the supernatant of splenocyte cultures. The protection engendered by this vaccination protocol was confirmed by 42% reduction in worm burden, 45% reduction in eggs per gram of hepatic tissue, 29% reduction in the number of granulomas per area, and 53% reduction in the granuloma fibrosis. Taken together, the data herein support the potential of surface-exposed GPI-anchored antigens from the S. mansoni tegument as vaccine candidate. Hindawi Publishing Corporation 2012 2012-08-15 /pmc/articles/PMC3426240/ /pubmed/22927873 http://dx.doi.org/10.1155/2012/962538 Text en Copyright © 2012 Vicente P. Martins et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Martins, Vicente P.
Pinheiro, Carina S.
Figueiredo, Barbara C. P.
Assis, Natan R. G.
Morais, Suellen B.
Caliari, Marcelo V.
Azevedo, Vasco
Castro-Borges, William
Wilson, R. Alan
Oliveira, Sergio C.
Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_full Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_fullStr Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_full_unstemmed Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_short Vaccination with Enzymatically Cleaved GPI-Anchored Proteins from Schistosoma mansoni Induces Protection against Challenge Infection
title_sort vaccination with enzymatically cleaved gpi-anchored proteins from schistosoma mansoni induces protection against challenge infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426240/
https://www.ncbi.nlm.nih.gov/pubmed/22927873
http://dx.doi.org/10.1155/2012/962538
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