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XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1

Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants an...

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Autores principales: Matsumoto, Ayumi, Huston, Sherry L, Killiny, Nabil, Igo, Michele M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Blackwell Publishing Inc 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426408/
https://www.ncbi.nlm.nih.gov/pubmed/22950010
http://dx.doi.org/10.1002/mbo3.6
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author Matsumoto, Ayumi
Huston, Sherry L
Killiny, Nabil
Igo, Michele M
author_facet Matsumoto, Ayumi
Huston, Sherry L
Killiny, Nabil
Igo, Michele M
author_sort Matsumoto, Ayumi
collection PubMed
description Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. Here, we describe our characterization of XatA, the X. fastidiosa autotransporter protein encoded by PD0528. XatA, which is classified as an AT-1 (classical) autotransporter, has a C-terminal β-barrel domain and a passenger domain composed of six tandem repeats of approximately 50 amino acids. Localization studies indicate that XatA is present in both the outer membrane and membrane vesicles and its passenger domain can be found in the supernatant. Moreover, XatA is important for X. fastidiosa autoaggregation and biofilm formation based on mutational analysis and the discovery that Escherichia coli expressing XatA acquire these traits. The xatA mutant also shows a significant decrease in Pierce's disease symptoms when inoculated into grapevines. Finally, X. fastidiosa homologs to XatA, which can be divided into three distinct groups based on synteny, form a single, well-supported clade, suggesting that they arose from a common ancestor.
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spelling pubmed-34264082012-08-29 XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1 Matsumoto, Ayumi Huston, Sherry L Killiny, Nabil Igo, Michele M Microbiologyopen Original Research Xylella fastidiosa Temecula1 is the causative agent of Pierce's disease of grapevine, which is spread by xylem-feeding insects. An important feature of the infection cycle is the ability of X. fastidiosa to colonize and interact with two distinct environments, the xylem of susceptible plants and the insect foregut. Here, we describe our characterization of XatA, the X. fastidiosa autotransporter protein encoded by PD0528. XatA, which is classified as an AT-1 (classical) autotransporter, has a C-terminal β-barrel domain and a passenger domain composed of six tandem repeats of approximately 50 amino acids. Localization studies indicate that XatA is present in both the outer membrane and membrane vesicles and its passenger domain can be found in the supernatant. Moreover, XatA is important for X. fastidiosa autoaggregation and biofilm formation based on mutational analysis and the discovery that Escherichia coli expressing XatA acquire these traits. The xatA mutant also shows a significant decrease in Pierce's disease symptoms when inoculated into grapevines. Finally, X. fastidiosa homologs to XatA, which can be divided into three distinct groups based on synteny, form a single, well-supported clade, suggesting that they arose from a common ancestor. Blackwell Publishing Inc 2012-03 /pmc/articles/PMC3426408/ /pubmed/22950010 http://dx.doi.org/10.1002/mbo3.6 Text en © 2012 The Authors. Published by Blackwell Publishing Ltd. http://creativecommons.org/licenses/by/2.5/ This is an open access article under the terms of the Creative Commons Attribution Non Commercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Research
Matsumoto, Ayumi
Huston, Sherry L
Killiny, Nabil
Igo, Michele M
XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
title XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
title_full XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
title_fullStr XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
title_full_unstemmed XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
title_short XatA, an AT-1 autotransporter important for the virulence of Xylella fastidiosa Temecula1
title_sort xata, an at-1 autotransporter important for the virulence of xylella fastidiosa temecula1
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426408/
https://www.ncbi.nlm.nih.gov/pubmed/22950010
http://dx.doi.org/10.1002/mbo3.6
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