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Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma

INTRODUCTION: Skeletal muscle trauma leads to severe functional deficits, which cannot be addressed by current treatment options. Our group could show the efficacy of local transplantation of mesenchymal stroma cells (MSCs) for the treatment of injured muscles. While local application of MSCs has pr...

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Autores principales: von Roth, Philipp, Duda, Georg N, Radojewski, Piotr, Preininger, Bernd, Strohschein, Kristin, Röhner, Eric, Perka, Carsten, Winkler, Tobias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426785/
https://www.ncbi.nlm.nih.gov/pubmed/22927895
http://dx.doi.org/10.2174/1874325001206010352
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author von Roth, Philipp
Duda, Georg N
Radojewski, Piotr
Preininger, Bernd
Strohschein, Kristin
Röhner, Eric
Perka, Carsten
Winkler, Tobias
author_facet von Roth, Philipp
Duda, Georg N
Radojewski, Piotr
Preininger, Bernd
Strohschein, Kristin
Röhner, Eric
Perka, Carsten
Winkler, Tobias
author_sort von Roth, Philipp
collection PubMed
description INTRODUCTION: Skeletal muscle trauma leads to severe functional deficits, which cannot be addressed by current treatment options. Our group could show the efficacy of local transplantation of mesenchymal stroma cells (MSCs) for the treatment of injured muscles. While local application of MSCs has proven to be effective, we hypothesized that a selective intra-arterial transplantation would lead to a better distribution of the cells and so improved physiological recovery of muscle function. MATERIALS AND METHODOLOGY: 18 female Sprague Dawley rats received an open crush trauma of the left soleus muscle. Autologous MSC were transduced using dsCOP-GFP and 2.5 x 10(6)cells were transplanted into the femoral artery of 9 animals one week after trauma. Control animals (n=9) received a saline injection. Cell tracking, analysis of tissue fibrosis and muscle force measurements were performed after 3 weeks. RESULTS: Systemic MSC-therapy improved the muscle force significantly compared to control (fast twitch: 82.4%, tetany: 61.6%, p = 0.02). The histological analysis showed no differences in the quantity of fibrotic tissue. Histological examination revealed no cells in the traumatized muscle tissue 21 days after transplantation. CONCLUSIONS: The present study demonstrated an effect of systemically administered MSCs in the treatment of skeletal muscle injuries. For possible future therapeutic approaches a systemic application of MSCs seems to present an alternative to a local administration. Such systemic treatment would be preferable since it allows functional improvement and possible cellular concentration at injury sites that are not easily accessible
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spelling pubmed-34267852012-08-27 Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma von Roth, Philipp Duda, Georg N Radojewski, Piotr Preininger, Bernd Strohschein, Kristin Röhner, Eric Perka, Carsten Winkler, Tobias Open Orthop J Article INTRODUCTION: Skeletal muscle trauma leads to severe functional deficits, which cannot be addressed by current treatment options. Our group could show the efficacy of local transplantation of mesenchymal stroma cells (MSCs) for the treatment of injured muscles. While local application of MSCs has proven to be effective, we hypothesized that a selective intra-arterial transplantation would lead to a better distribution of the cells and so improved physiological recovery of muscle function. MATERIALS AND METHODOLOGY: 18 female Sprague Dawley rats received an open crush trauma of the left soleus muscle. Autologous MSC were transduced using dsCOP-GFP and 2.5 x 10(6)cells were transplanted into the femoral artery of 9 animals one week after trauma. Control animals (n=9) received a saline injection. Cell tracking, analysis of tissue fibrosis and muscle force measurements were performed after 3 weeks. RESULTS: Systemic MSC-therapy improved the muscle force significantly compared to control (fast twitch: 82.4%, tetany: 61.6%, p = 0.02). The histological analysis showed no differences in the quantity of fibrotic tissue. Histological examination revealed no cells in the traumatized muscle tissue 21 days after transplantation. CONCLUSIONS: The present study demonstrated an effect of systemically administered MSCs in the treatment of skeletal muscle injuries. For possible future therapeutic approaches a systemic application of MSCs seems to present an alternative to a local administration. Such systemic treatment would be preferable since it allows functional improvement and possible cellular concentration at injury sites that are not easily accessible Bentham Open 2012-08-10 /pmc/articles/PMC3426785/ /pubmed/22927895 http://dx.doi.org/10.2174/1874325001206010352 Text en © von Roth et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
von Roth, Philipp
Duda, Georg N
Radojewski, Piotr
Preininger, Bernd
Strohschein, Kristin
Röhner, Eric
Perka, Carsten
Winkler, Tobias
Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma
title Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma
title_full Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma
title_fullStr Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma
title_full_unstemmed Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma
title_short Intra-Arterial MSC Transplantation Restores Functional Capacity After Skeletal Muscle Trauma
title_sort intra-arterial msc transplantation restores functional capacity after skeletal muscle trauma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426785/
https://www.ncbi.nlm.nih.gov/pubmed/22927895
http://dx.doi.org/10.2174/1874325001206010352
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