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Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections

AIM: This study compared the perfomance of the RNA-based EndoPredict multigene test on core biopsies and surgical breast cancer specimens and analysed the influence of biopsy-induced tissue injuries on the test result. METHODS: 80 formalin-fixed paraffin-embedded samples comprising paired biopsies a...

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Autores principales: Müller, Berit Maria, Brase, Jan C, Haufe, Franziska, Weber, Karsten E, Budzies, Jan, Petry, Christoph, Prinzler, Judith, Kronenwett, Ralf, Dietel, Manfred, Denkert, Carsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426896/
https://www.ncbi.nlm.nih.gov/pubmed/22447922
http://dx.doi.org/10.1136/jclinpath-2012-200716
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author Müller, Berit Maria
Brase, Jan C
Haufe, Franziska
Weber, Karsten E
Budzies, Jan
Petry, Christoph
Prinzler, Judith
Kronenwett, Ralf
Dietel, Manfred
Denkert, Carsten
author_facet Müller, Berit Maria
Brase, Jan C
Haufe, Franziska
Weber, Karsten E
Budzies, Jan
Petry, Christoph
Prinzler, Judith
Kronenwett, Ralf
Dietel, Manfred
Denkert, Carsten
author_sort Müller, Berit Maria
collection PubMed
description AIM: This study compared the perfomance of the RNA-based EndoPredict multigene test on core biopsies and surgical breast cancer specimens and analysed the influence of biopsy-induced tissue injuries on the test result. METHODS: 80 formalin-fixed paraffin-embedded samples comprising paired biopsies and surgical specimens from 40 ER-positive, HER2-negative patients were evaluated. Total RNA was extracted and the EndoPredict score was determined. RESULTS: RNA yield was considerably lower in core biopsies, but sufficient to measure the assay in all samples. The EndoPredict score was highly correlated between paired samples (Pearson r=0.92), with an excellent concordance of classification into a low or high risk of metastasis (overall agreement 95%). CONCLUSIONS: The measurements are comparable between core biopsies and surgical sections, which suggest that the EndoPredict assay can be performed on core biopsy tissue. Inflammatory changes induced by presurgical biopsies had no significant effect on the RNA-based risk assessment in surgical specimens.
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spelling pubmed-34268962012-08-24 Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections Müller, Berit Maria Brase, Jan C Haufe, Franziska Weber, Karsten E Budzies, Jan Petry, Christoph Prinzler, Judith Kronenwett, Ralf Dietel, Manfred Denkert, Carsten J Clin Pathol Short Report AIM: This study compared the perfomance of the RNA-based EndoPredict multigene test on core biopsies and surgical breast cancer specimens and analysed the influence of biopsy-induced tissue injuries on the test result. METHODS: 80 formalin-fixed paraffin-embedded samples comprising paired biopsies and surgical specimens from 40 ER-positive, HER2-negative patients were evaluated. Total RNA was extracted and the EndoPredict score was determined. RESULTS: RNA yield was considerably lower in core biopsies, but sufficient to measure the assay in all samples. The EndoPredict score was highly correlated between paired samples (Pearson r=0.92), with an excellent concordance of classification into a low or high risk of metastasis (overall agreement 95%). CONCLUSIONS: The measurements are comparable between core biopsies and surgical sections, which suggest that the EndoPredict assay can be performed on core biopsy tissue. Inflammatory changes induced by presurgical biopsies had no significant effect on the RNA-based risk assessment in surgical specimens. BMJ Group 2012-03-23 2012-07 /pmc/articles/PMC3426896/ /pubmed/22447922 http://dx.doi.org/10.1136/jclinpath-2012-200716 Text en © 2012, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.
spellingShingle Short Report
Müller, Berit Maria
Brase, Jan C
Haufe, Franziska
Weber, Karsten E
Budzies, Jan
Petry, Christoph
Prinzler, Judith
Kronenwett, Ralf
Dietel, Manfred
Denkert, Carsten
Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
title Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
title_full Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
title_fullStr Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
title_full_unstemmed Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
title_short Comparison of the RNA-based EndoPredict multigene test between core biopsies and corresponding surgical breast cancer sections
title_sort comparison of the rna-based endopredict multigene test between core biopsies and corresponding surgical breast cancer sections
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3426896/
https://www.ncbi.nlm.nih.gov/pubmed/22447922
http://dx.doi.org/10.1136/jclinpath-2012-200716
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