Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China

Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further...

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Detalles Bibliográficos
Autores principales: Zhu, Huili, Zhao, Jinfeng, Wang, Rongjun, Zhang, Longxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427244/
https://www.ncbi.nlm.nih.gov/pubmed/22937078
http://dx.doi.org/10.1371/journal.pone.0043682
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author Zhu, Huili
Zhao, Jinfeng
Wang, Rongjun
Zhang, Longxian
author_facet Zhu, Huili
Zhao, Jinfeng
Wang, Rongjun
Zhang, Longxian
author_sort Zhu, Huili
collection PubMed
description Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan.
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spelling pubmed-34272442012-08-30 Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China Zhu, Huili Zhao, Jinfeng Wang, Rongjun Zhang, Longxian PLoS One Research Article Two Cryptosporidium isolates from separate infants suffering from diarrhea were obtained from a hospital in Zhengzhou, China and were genotyped by PCR amplification and sequence analysis of the small-subunit ribosomal RNA (rRNA) (SSU rRNA), 70-kDa heat shock protein (HSP70), and actin genes. Further subtyping was performed by PCR amplification and sequence analysis of the 60-kDa glycoprotein (gp60) gene. Both the isolates were identified as Cryptosporidium hominis subtype IdA21, a rare subtype previously found only in a human immunodeficiency virus-infected child in South Africa and another child in Jordan. Public Library of Science 2012-08-24 /pmc/articles/PMC3427244/ /pubmed/22937078 http://dx.doi.org/10.1371/journal.pone.0043682 Text en © 2012 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhu, Huili
Zhao, Jinfeng
Wang, Rongjun
Zhang, Longxian
Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China
title Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China
title_full Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China
title_fullStr Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China
title_full_unstemmed Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China
title_short Molecular Identification of a Rare Subtype of Cryptosporidium hominis in Infants in China
title_sort molecular identification of a rare subtype of cryptosporidium hominis in infants in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427244/
https://www.ncbi.nlm.nih.gov/pubmed/22937078
http://dx.doi.org/10.1371/journal.pone.0043682
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AT zhanglongxian molecularidentificationofararesubtypeofcryptosporidiumhominisininfantsinchina

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