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Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan

We present the first comprehensive analysis of Mycobacterium tuberculosis isolates circulating in the Kaohsiung region of southern Taiwan. The major spoligotypes found in the 224 isolates studied were Beijing lineages (n = 97; 43.3%), EAI lineages (n = 72; 32.1%) and Haarlem lineages (n = 18; 8.0%)....

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Autores principales: Chang, Jia-Ru, Chen, Yih-Yuan, Huang, Tsi-Shu, Huang, Wei-Feng, Kuo, Shu-Chen, Tseng, Fan-Chen, Su, Ih-Jen, Lin, Chien-Hsing, Chen, Yao-Shen, Sun, Jun-Ren, Chiueh, Tzong-Shi, Dou, Horng-Yunn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427295/
https://www.ncbi.nlm.nih.gov/pubmed/22937008
http://dx.doi.org/10.1371/journal.pone.0043018
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author Chang, Jia-Ru
Chen, Yih-Yuan
Huang, Tsi-Shu
Huang, Wei-Feng
Kuo, Shu-Chen
Tseng, Fan-Chen
Su, Ih-Jen
Lin, Chien-Hsing
Chen, Yao-Shen
Sun, Jun-Ren
Chiueh, Tzong-Shi
Dou, Horng-Yunn
author_facet Chang, Jia-Ru
Chen, Yih-Yuan
Huang, Tsi-Shu
Huang, Wei-Feng
Kuo, Shu-Chen
Tseng, Fan-Chen
Su, Ih-Jen
Lin, Chien-Hsing
Chen, Yao-Shen
Sun, Jun-Ren
Chiueh, Tzong-Shi
Dou, Horng-Yunn
author_sort Chang, Jia-Ru
collection PubMed
description We present the first comprehensive analysis of Mycobacterium tuberculosis isolates circulating in the Kaohsiung region of southern Taiwan. The major spoligotypes found in the 224 isolates studied were Beijing lineages (n = 97; 43.3%), EAI lineages (n = 72; 32.1%) and Haarlem lineages (n = 18; 8.0%). By 24 MIRU-VNTR typing, 174 patterns were identified, including 24 clusters of 74 isolates and 150 unique patterns. The combination of spoligotyping and 12-MIRU-VNTR revealed that 129 (57.6%) of the 224 isolates were clustered in 18 genotypes. Moreover, 63.6% (7/11) of infected persons younger than 30 years had a Beijing strain, which could suggest recent spread among younger persons by this family of TB strains in Kaohsiung. Among the 94 Beijing family (SIT1, SIT250 and SIT1674) isolates further analyzed for SNPs by mass spectrometry, the most frequent strain found was ST10 (n = 49; 52%), followed by ST22 (n = 17; 18%) and ST19 (n = 11; 12%). Among the EAI-Manila family isolates analyzed by region deletion-based subtyping, the most frequent strain found was RD type 1 (n = 63; 87.5%), followed by RD type 2 (n = 9; 12.5%). In our previous study, the proportion of modern Beijing strains (52.5%) in northern Taiwan was significantly higher than the proportion of EAI strains (11%). In contrast, in the present study, EAI strains comprised up to 32% of Beijing strains in southern Taiwan. In conclusion, both ‘modern’ (Beijing) and ‘ancient’ (EAI) M. tuberculosis strains are prevalent in the Kaohsiung region, perhaps suggesting that both strains are somehow more adapted to southern Taiwan. It will be interesting to investigate the dynamics of the lineage composition by different selection pressures.
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spelling pubmed-34272952012-08-30 Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan Chang, Jia-Ru Chen, Yih-Yuan Huang, Tsi-Shu Huang, Wei-Feng Kuo, Shu-Chen Tseng, Fan-Chen Su, Ih-Jen Lin, Chien-Hsing Chen, Yao-Shen Sun, Jun-Ren Chiueh, Tzong-Shi Dou, Horng-Yunn PLoS One Research Article We present the first comprehensive analysis of Mycobacterium tuberculosis isolates circulating in the Kaohsiung region of southern Taiwan. The major spoligotypes found in the 224 isolates studied were Beijing lineages (n = 97; 43.3%), EAI lineages (n = 72; 32.1%) and Haarlem lineages (n = 18; 8.0%). By 24 MIRU-VNTR typing, 174 patterns were identified, including 24 clusters of 74 isolates and 150 unique patterns. The combination of spoligotyping and 12-MIRU-VNTR revealed that 129 (57.6%) of the 224 isolates were clustered in 18 genotypes. Moreover, 63.6% (7/11) of infected persons younger than 30 years had a Beijing strain, which could suggest recent spread among younger persons by this family of TB strains in Kaohsiung. Among the 94 Beijing family (SIT1, SIT250 and SIT1674) isolates further analyzed for SNPs by mass spectrometry, the most frequent strain found was ST10 (n = 49; 52%), followed by ST22 (n = 17; 18%) and ST19 (n = 11; 12%). Among the EAI-Manila family isolates analyzed by region deletion-based subtyping, the most frequent strain found was RD type 1 (n = 63; 87.5%), followed by RD type 2 (n = 9; 12.5%). In our previous study, the proportion of modern Beijing strains (52.5%) in northern Taiwan was significantly higher than the proportion of EAI strains (11%). In contrast, in the present study, EAI strains comprised up to 32% of Beijing strains in southern Taiwan. In conclusion, both ‘modern’ (Beijing) and ‘ancient’ (EAI) M. tuberculosis strains are prevalent in the Kaohsiung region, perhaps suggesting that both strains are somehow more adapted to southern Taiwan. It will be interesting to investigate the dynamics of the lineage composition by different selection pressures. Public Library of Science 2012-08-24 /pmc/articles/PMC3427295/ /pubmed/22937008 http://dx.doi.org/10.1371/journal.pone.0043018 Text en © 2012 Chang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chang, Jia-Ru
Chen, Yih-Yuan
Huang, Tsi-Shu
Huang, Wei-Feng
Kuo, Shu-Chen
Tseng, Fan-Chen
Su, Ih-Jen
Lin, Chien-Hsing
Chen, Yao-Shen
Sun, Jun-Ren
Chiueh, Tzong-Shi
Dou, Horng-Yunn
Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan
title Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan
title_full Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan
title_fullStr Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan
title_full_unstemmed Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan
title_short Clonal Expansion of Both Modern and Ancient Genotypes of Mycobacterium tuberculosis in Southern Taiwan
title_sort clonal expansion of both modern and ancient genotypes of mycobacterium tuberculosis in southern taiwan
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427295/
https://www.ncbi.nlm.nih.gov/pubmed/22937008
http://dx.doi.org/10.1371/journal.pone.0043018
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