Expanding the set of rhodococcal Baeyer–Villiger monooxygenases by high-throughput cloning, expression and substrate screening

To expand the available set of Baeyer–Villiger monooxygenases (BVMOs), we have created expression constructs for producing 22 Type I BVMOs that are present in the genome of Rhodococcus jostii RHA1. Each BVMO has been probed with a large panel of potential substrates. Except for testing their substra...

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Detalles Bibliográficos
Autores principales: Riebel, A., Dudek, H. M., de Gonzalo, G., Stepniak, P., Rychlewski, L., Fraaije, M. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Biotechnologically Relevant Enzymes and Proteins
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427485/
https://www.ncbi.nlm.nih.gov/pubmed/22218769
http://dx.doi.org/10.1007/s00253-011-3823-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427485/
https://www.ncbi.nlm.nih.gov/pubmed/22218769
http://dx.doi.org/10.1007/s00253-011-3823-0

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