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Expanding the set of rhodococcal Baeyer–Villiger monooxygenases by high-throughput cloning, expression and substrate screening
To expand the available set of Baeyer–Villiger monooxygenases (BVMOs), we have created expression constructs for producing 22 Type I BVMOs that are present in the genome of Rhodococcus jostii RHA1. Each BVMO has been probed with a large panel of potential substrates. Except for testing their substra...
Autores principales: | Riebel, A., Dudek, H. M., de Gonzalo, G., Stepniak, P., Rychlewski, L., Fraaije, M. W. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer-Verlag
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427485/ https://www.ncbi.nlm.nih.gov/pubmed/22218769 http://dx.doi.org/10.1007/s00253-011-3823-0 |
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