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Preservation of 5300 year old red blood cells in the Iceman
Changes in elasticity and structures of red blood cells (RBCs) are important indicators of disease, and this makes them interesting for medical studies. In forensics, blood analyses represent a crucial part of crime scene investigations. For these reasons, the recovery and analysis of blood cells fr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427508/ https://www.ncbi.nlm.nih.gov/pubmed/22552923 http://dx.doi.org/10.1098/rsif.2012.0174 |
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author | Janko, Marek Stark, Robert W. Zink, Albert |
author_facet | Janko, Marek Stark, Robert W. Zink, Albert |
author_sort | Janko, Marek |
collection | PubMed |
description | Changes in elasticity and structures of red blood cells (RBCs) are important indicators of disease, and this makes them interesting for medical studies. In forensics, blood analyses represent a crucial part of crime scene investigations. For these reasons, the recovery and analysis of blood cells from ancient tissues is of major interest. In this study, we show that RBCs were preserved in Iceman tissue samples for more than 5000 years. The morphological and molecular composition of the blood corpuscle is verified by atomic force microscope and Raman spectroscopy measurements. The cell size and shape approximated those of healthy, dried, recent RBCs. Raman spectra of the ancient corpuscle revealed bands that are characteristic of haemoglobin. Additional vibrational modes typical for other proteinaceous fragments, possibly fibrin, suggested the formation of a blood clot. The band intensities, however, were approximately an order of magnitude weaker than those of recent RBCs. This fact points to a decrease in the RBC-specific metalloprotein haemoglobin and, thus, to a degradation of the cells. Together, the results show the preservation of RBCs in the 5000 year old mummy tissue and give the first insights into their degradation. |
format | Online Article Text |
id | pubmed-3427508 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-34275082012-08-28 Preservation of 5300 year old red blood cells in the Iceman Janko, Marek Stark, Robert W. Zink, Albert J R Soc Interface Research Articles Changes in elasticity and structures of red blood cells (RBCs) are important indicators of disease, and this makes them interesting for medical studies. In forensics, blood analyses represent a crucial part of crime scene investigations. For these reasons, the recovery and analysis of blood cells from ancient tissues is of major interest. In this study, we show that RBCs were preserved in Iceman tissue samples for more than 5000 years. The morphological and molecular composition of the blood corpuscle is verified by atomic force microscope and Raman spectroscopy measurements. The cell size and shape approximated those of healthy, dried, recent RBCs. Raman spectra of the ancient corpuscle revealed bands that are characteristic of haemoglobin. Additional vibrational modes typical for other proteinaceous fragments, possibly fibrin, suggested the formation of a blood clot. The band intensities, however, were approximately an order of magnitude weaker than those of recent RBCs. This fact points to a decrease in the RBC-specific metalloprotein haemoglobin and, thus, to a degradation of the cells. Together, the results show the preservation of RBCs in the 5000 year old mummy tissue and give the first insights into their degradation. The Royal Society 2012-10-07 2012-05-02 /pmc/articles/PMC3427508/ /pubmed/22552923 http://dx.doi.org/10.1098/rsif.2012.0174 Text en This journal is © 2012 The Royal Society http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Janko, Marek Stark, Robert W. Zink, Albert Preservation of 5300 year old red blood cells in the Iceman |
title | Preservation of 5300 year old red blood cells in the Iceman |
title_full | Preservation of 5300 year old red blood cells in the Iceman |
title_fullStr | Preservation of 5300 year old red blood cells in the Iceman |
title_full_unstemmed | Preservation of 5300 year old red blood cells in the Iceman |
title_short | Preservation of 5300 year old red blood cells in the Iceman |
title_sort | preservation of 5300 year old red blood cells in the iceman |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3427508/ https://www.ncbi.nlm.nih.gov/pubmed/22552923 http://dx.doi.org/10.1098/rsif.2012.0174 |
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