Genome-scale promoter engineering by Co-Selection MAGE

Multiplex Automated Genome Engineering (MAGE) employs short oligonucleotides to scarlessly modify genomes. However, insertions of >10 bases are still inefficient, but can be improved substantially by selection of highly modified chromosomes. Here, we describe Co-Selection MAGE (CoS-MAGE) to optim...

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Detalles Bibliográficos
Autores principales: Wang, Harris H., Kim, Hwangbeom, Cong, Le, Jeong, Jaehwan, Bang, Duhee, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428217/
https://www.ncbi.nlm.nih.gov/pubmed/22484848
http://dx.doi.org/10.1038/nmeth.1971
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author Wang, Harris H.
Kim, Hwangbeom
Cong, Le
Jeong, Jaehwan
Bang, Duhee
Church, George M.
author_facet Wang, Harris H.
Kim, Hwangbeom
Cong, Le
Jeong, Jaehwan
Bang, Duhee
Church, George M.
author_sort Wang, Harris H.
collection PubMed
description Multiplex Automated Genome Engineering (MAGE) employs short oligonucleotides to scarlessly modify genomes. However, insertions of >10 bases are still inefficient, but can be improved substantially by selection of highly modified chromosomes. Here, we describe Co-Selection MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks.
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spelling pubmed-34282172012-12-01 Genome-scale promoter engineering by Co-Selection MAGE Wang, Harris H. Kim, Hwangbeom Cong, Le Jeong, Jaehwan Bang, Duhee Church, George M. Nat Methods Article Multiplex Automated Genome Engineering (MAGE) employs short oligonucleotides to scarlessly modify genomes. However, insertions of >10 bases are still inefficient, but can be improved substantially by selection of highly modified chromosomes. Here, we describe Co-Selection MAGE (CoS-MAGE) to optimize biosynthesis of aromatic amino acid derivatives by combinatorially inserting multiple T7 promoters simultaneously into 12 genomic operons. Promoter libraries can be quickly generated to study gain-of-function epistatic interactions in gene networks. 2012-04-08 2012-06 /pmc/articles/PMC3428217/ /pubmed/22484848 http://dx.doi.org/10.1038/nmeth.1971 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Wang, Harris H.
Kim, Hwangbeom
Cong, Le
Jeong, Jaehwan
Bang, Duhee
Church, George M.
Genome-scale promoter engineering by Co-Selection MAGE
title Genome-scale promoter engineering by Co-Selection MAGE
title_full Genome-scale promoter engineering by Co-Selection MAGE
title_fullStr Genome-scale promoter engineering by Co-Selection MAGE
title_full_unstemmed Genome-scale promoter engineering by Co-Selection MAGE
title_short Genome-scale promoter engineering by Co-Selection MAGE
title_sort genome-scale promoter engineering by co-selection mage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428217/
https://www.ncbi.nlm.nih.gov/pubmed/22484848
http://dx.doi.org/10.1038/nmeth.1971
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