Cargando…

Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors

The tolerant mechanism of yeast to the combination of three inhibitors (furfural, phenol and acetic acid) was investigated using 2-DE combined with MALDI-TOF/TOF-MS. The stress response and detoxification related proteins (e.g., Ahp1p, Hsp26p) were expressed higher in the tolerant yeast than in the...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Ming-Zhu, Wang, Xin, Liu, Wei, Cheng, Jing-Sheng, Yang, Yang, Yuan, Ying-Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428360/
https://www.ncbi.nlm.nih.gov/pubmed/22952687
http://dx.doi.org/10.1371/journal.pone.0043474
_version_ 1782241694341660672
author Ding, Ming-Zhu
Wang, Xin
Liu, Wei
Cheng, Jing-Sheng
Yang, Yang
Yuan, Ying-Jin
author_facet Ding, Ming-Zhu
Wang, Xin
Liu, Wei
Cheng, Jing-Sheng
Yang, Yang
Yuan, Ying-Jin
author_sort Ding, Ming-Zhu
collection PubMed
description The tolerant mechanism of yeast to the combination of three inhibitors (furfural, phenol and acetic acid) was investigated using 2-DE combined with MALDI-TOF/TOF-MS. The stress response and detoxification related proteins (e.g., Ahp1p, Hsp26p) were expressed higher in the tolerant yeast than in the parental yeast. The expressions of most nitrogen metabolism related proteins (e.g. Gdh1p, Met1p) were higher in the parental yeast, indicating that the tolerant yeast decreases its nitrogen metabolism rate to reserve energy, and possesses high resistance to the stress of combined inhibitors. Furthermore, upon exposure to the inhibitors, the proteins related to protein folding, degradation and translation (e.g., Ssc1p, Ubp14p, Efb1p) were all significantly affected, and the oxidative stress related proteins (e.g., Ahp1p, Grx1p) were increased. Knockdown of genes related to the oxidative stress and unfolded protein response (Grx1, Gre2, Asc1) significantly decreased the tolerance of yeast to inhibitors, which further suggested that yeast responded to the inhibitors mainly by inducing unfolded protein response. This study reveals that increasing the detoxification and tolerating oxidative stress, and/or decreasing the nitrogen metabolism would be promising strategies in developing more tolerant strains to the multiple inhibitors in lignocellulose hydrolysates.
format Online
Article
Text
id pubmed-3428360
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-34283602012-09-05 Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors Ding, Ming-Zhu Wang, Xin Liu, Wei Cheng, Jing-Sheng Yang, Yang Yuan, Ying-Jin PLoS One Research Article The tolerant mechanism of yeast to the combination of three inhibitors (furfural, phenol and acetic acid) was investigated using 2-DE combined with MALDI-TOF/TOF-MS. The stress response and detoxification related proteins (e.g., Ahp1p, Hsp26p) were expressed higher in the tolerant yeast than in the parental yeast. The expressions of most nitrogen metabolism related proteins (e.g. Gdh1p, Met1p) were higher in the parental yeast, indicating that the tolerant yeast decreases its nitrogen metabolism rate to reserve energy, and possesses high resistance to the stress of combined inhibitors. Furthermore, upon exposure to the inhibitors, the proteins related to protein folding, degradation and translation (e.g., Ssc1p, Ubp14p, Efb1p) were all significantly affected, and the oxidative stress related proteins (e.g., Ahp1p, Grx1p) were increased. Knockdown of genes related to the oxidative stress and unfolded protein response (Grx1, Gre2, Asc1) significantly decreased the tolerance of yeast to inhibitors, which further suggested that yeast responded to the inhibitors mainly by inducing unfolded protein response. This study reveals that increasing the detoxification and tolerating oxidative stress, and/or decreasing the nitrogen metabolism would be promising strategies in developing more tolerant strains to the multiple inhibitors in lignocellulose hydrolysates. Public Library of Science 2012-08-27 /pmc/articles/PMC3428360/ /pubmed/22952687 http://dx.doi.org/10.1371/journal.pone.0043474 Text en © 2012 Ding et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ding, Ming-Zhu
Wang, Xin
Liu, Wei
Cheng, Jing-Sheng
Yang, Yang
Yuan, Ying-Jin
Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
title Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
title_full Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
title_fullStr Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
title_full_unstemmed Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
title_short Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
title_sort proteomic research reveals the stress response and detoxification of yeast to combined inhibitors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428360/
https://www.ncbi.nlm.nih.gov/pubmed/22952687
http://dx.doi.org/10.1371/journal.pone.0043474
work_keys_str_mv AT dingmingzhu proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors
AT wangxin proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors
AT liuwei proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors
AT chengjingsheng proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors
AT yangyang proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors
AT yuanyingjin proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors