Cargando…
Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors
The tolerant mechanism of yeast to the combination of three inhibitors (furfural, phenol and acetic acid) was investigated using 2-DE combined with MALDI-TOF/TOF-MS. The stress response and detoxification related proteins (e.g., Ahp1p, Hsp26p) were expressed higher in the tolerant yeast than in the...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428360/ https://www.ncbi.nlm.nih.gov/pubmed/22952687 http://dx.doi.org/10.1371/journal.pone.0043474 |
_version_ | 1782241694341660672 |
---|---|
author | Ding, Ming-Zhu Wang, Xin Liu, Wei Cheng, Jing-Sheng Yang, Yang Yuan, Ying-Jin |
author_facet | Ding, Ming-Zhu Wang, Xin Liu, Wei Cheng, Jing-Sheng Yang, Yang Yuan, Ying-Jin |
author_sort | Ding, Ming-Zhu |
collection | PubMed |
description | The tolerant mechanism of yeast to the combination of three inhibitors (furfural, phenol and acetic acid) was investigated using 2-DE combined with MALDI-TOF/TOF-MS. The stress response and detoxification related proteins (e.g., Ahp1p, Hsp26p) were expressed higher in the tolerant yeast than in the parental yeast. The expressions of most nitrogen metabolism related proteins (e.g. Gdh1p, Met1p) were higher in the parental yeast, indicating that the tolerant yeast decreases its nitrogen metabolism rate to reserve energy, and possesses high resistance to the stress of combined inhibitors. Furthermore, upon exposure to the inhibitors, the proteins related to protein folding, degradation and translation (e.g., Ssc1p, Ubp14p, Efb1p) were all significantly affected, and the oxidative stress related proteins (e.g., Ahp1p, Grx1p) were increased. Knockdown of genes related to the oxidative stress and unfolded protein response (Grx1, Gre2, Asc1) significantly decreased the tolerance of yeast to inhibitors, which further suggested that yeast responded to the inhibitors mainly by inducing unfolded protein response. This study reveals that increasing the detoxification and tolerating oxidative stress, and/or decreasing the nitrogen metabolism would be promising strategies in developing more tolerant strains to the multiple inhibitors in lignocellulose hydrolysates. |
format | Online Article Text |
id | pubmed-3428360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34283602012-09-05 Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors Ding, Ming-Zhu Wang, Xin Liu, Wei Cheng, Jing-Sheng Yang, Yang Yuan, Ying-Jin PLoS One Research Article The tolerant mechanism of yeast to the combination of three inhibitors (furfural, phenol and acetic acid) was investigated using 2-DE combined with MALDI-TOF/TOF-MS. The stress response and detoxification related proteins (e.g., Ahp1p, Hsp26p) were expressed higher in the tolerant yeast than in the parental yeast. The expressions of most nitrogen metabolism related proteins (e.g. Gdh1p, Met1p) were higher in the parental yeast, indicating that the tolerant yeast decreases its nitrogen metabolism rate to reserve energy, and possesses high resistance to the stress of combined inhibitors. Furthermore, upon exposure to the inhibitors, the proteins related to protein folding, degradation and translation (e.g., Ssc1p, Ubp14p, Efb1p) were all significantly affected, and the oxidative stress related proteins (e.g., Ahp1p, Grx1p) were increased. Knockdown of genes related to the oxidative stress and unfolded protein response (Grx1, Gre2, Asc1) significantly decreased the tolerance of yeast to inhibitors, which further suggested that yeast responded to the inhibitors mainly by inducing unfolded protein response. This study reveals that increasing the detoxification and tolerating oxidative stress, and/or decreasing the nitrogen metabolism would be promising strategies in developing more tolerant strains to the multiple inhibitors in lignocellulose hydrolysates. Public Library of Science 2012-08-27 /pmc/articles/PMC3428360/ /pubmed/22952687 http://dx.doi.org/10.1371/journal.pone.0043474 Text en © 2012 Ding et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ding, Ming-Zhu Wang, Xin Liu, Wei Cheng, Jing-Sheng Yang, Yang Yuan, Ying-Jin Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors |
title | Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors |
title_full | Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors |
title_fullStr | Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors |
title_full_unstemmed | Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors |
title_short | Proteomic Research Reveals the Stress Response and Detoxification of Yeast to Combined Inhibitors |
title_sort | proteomic research reveals the stress response and detoxification of yeast to combined inhibitors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3428360/ https://www.ncbi.nlm.nih.gov/pubmed/22952687 http://dx.doi.org/10.1371/journal.pone.0043474 |
work_keys_str_mv | AT dingmingzhu proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors AT wangxin proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors AT liuwei proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors AT chengjingsheng proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors AT yangyang proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors AT yuanyingjin proteomicresearchrevealsthestressresponseanddetoxificationofyeasttocombinedinhibitors |