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Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100

Two artificial intelligence techniques, namely artificial neural network (ANN) and genetic algorithm (GA) were combined to be used as a tool for optimizing the covalent immobilization of cellulase on a smart polymer, Eudragit L-100. 1-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide (EDC) concentration...

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Autores principales: Zhang, Yu, Xu, Jing-Liang, Yuan, Zhen-Hong, Qi, Wei, Liu, Yun-Yun, He, Min-Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430214/
https://www.ncbi.nlm.nih.gov/pubmed/22942683
http://dx.doi.org/10.3390/ijms13077952
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author Zhang, Yu
Xu, Jing-Liang
Yuan, Zhen-Hong
Qi, Wei
Liu, Yun-Yun
He, Min-Chao
author_facet Zhang, Yu
Xu, Jing-Liang
Yuan, Zhen-Hong
Qi, Wei
Liu, Yun-Yun
He, Min-Chao
author_sort Zhang, Yu
collection PubMed
description Two artificial intelligence techniques, namely artificial neural network (ANN) and genetic algorithm (GA) were combined to be used as a tool for optimizing the covalent immobilization of cellulase on a smart polymer, Eudragit L-100. 1-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide (EDC) concentration, N-hydroxysuccinimide (NHS) concentration and coupling time were taken as independent variables, and immobilization efficiency was taken as the response. The data of the central composite design were used to train ANN by back-propagation algorithm, and the result showed that the trained ANN fitted the data accurately (correlation coefficient R(2) = 0.99). Then a maximum immobilization efficiency of 88.76% was searched by genetic algorithm at a EDC concentration of 0.44%, NHS concentration of 0.37% and a coupling time of 2.22 h, where the experimental value was 87.97 ± 6.45%. The application of ANN based optimization by GA is quite successful.
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spelling pubmed-34302142012-08-31 Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100 Zhang, Yu Xu, Jing-Liang Yuan, Zhen-Hong Qi, Wei Liu, Yun-Yun He, Min-Chao Int J Mol Sci Article Two artificial intelligence techniques, namely artificial neural network (ANN) and genetic algorithm (GA) were combined to be used as a tool for optimizing the covalent immobilization of cellulase on a smart polymer, Eudragit L-100. 1-Ethyl-3-(3-dimethyllaminopropyl) carbodiimide (EDC) concentration, N-hydroxysuccinimide (NHS) concentration and coupling time were taken as independent variables, and immobilization efficiency was taken as the response. The data of the central composite design were used to train ANN by back-propagation algorithm, and the result showed that the trained ANN fitted the data accurately (correlation coefficient R(2) = 0.99). Then a maximum immobilization efficiency of 88.76% was searched by genetic algorithm at a EDC concentration of 0.44%, NHS concentration of 0.37% and a coupling time of 2.22 h, where the experimental value was 87.97 ± 6.45%. The application of ANN based optimization by GA is quite successful. Molecular Diversity Preservation International (MDPI) 2012-06-26 /pmc/articles/PMC3430214/ /pubmed/22942683 http://dx.doi.org/10.3390/ijms13077952 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Zhang, Yu
Xu, Jing-Liang
Yuan, Zhen-Hong
Qi, Wei
Liu, Yun-Yun
He, Min-Chao
Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100
title Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100
title_full Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100
title_fullStr Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100
title_full_unstemmed Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100
title_short Artificial Intelligence Techniques to Optimize the EDC/NHS-Mediated Immobilization of Cellulase on Eudragit L-100
title_sort artificial intelligence techniques to optimize the edc/nhs-mediated immobilization of cellulase on eudragit l-100
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430214/
https://www.ncbi.nlm.nih.gov/pubmed/22942683
http://dx.doi.org/10.3390/ijms13077952
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