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X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure
Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report t...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430646/ https://www.ncbi.nlm.nih.gov/pubmed/22952569 http://dx.doi.org/10.1371/journal.pone.0035691 |
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author | Langer, Max Pacureanu, Alexandra Suhonen, Heikki Grimal, Quentin Cloetens, Peter Peyrin, Françoise |
author_facet | Langer, Max Pacureanu, Alexandra Suhonen, Heikki Grimal, Quentin Cloetens, Peter Peyrin, Françoise |
author_sort | Langer, Max |
collection | PubMed |
description | Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report the investigation of the human bone ultrastructure with X-ray phase nanotomography, which now provides the required sensitivity, spatial resolution and field of view. The 3D organization of the lacuno-canalicular network is studied in detail over several cells in osteonal and interstitial tissue. Nanoscale density variations are revealed and show that the cement line separating these tissues is hypermineralized. Finally, we show that the collagen fibers are organized as a twisted plywood structure in 3D. |
format | Online Article Text |
id | pubmed-3430646 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34306462012-09-05 X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure Langer, Max Pacureanu, Alexandra Suhonen, Heikki Grimal, Quentin Cloetens, Peter Peyrin, Françoise PLoS One Research Article Bone strength and failure are increasingly thought to be due to ultrastructural properties, such as the morphology of the lacuno-canalicular network, the collagen fiber orientation and the mineralization on the nanoscale. However, these properties have not been studied in 3D so far. Here we report the investigation of the human bone ultrastructure with X-ray phase nanotomography, which now provides the required sensitivity, spatial resolution and field of view. The 3D organization of the lacuno-canalicular network is studied in detail over several cells in osteonal and interstitial tissue. Nanoscale density variations are revealed and show that the cement line separating these tissues is hypermineralized. Finally, we show that the collagen fibers are organized as a twisted plywood structure in 3D. Public Library of Science 2012-08-29 /pmc/articles/PMC3430646/ /pubmed/22952569 http://dx.doi.org/10.1371/journal.pone.0035691 Text en © 2012 Langer et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Langer, Max Pacureanu, Alexandra Suhonen, Heikki Grimal, Quentin Cloetens, Peter Peyrin, Françoise X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure |
title | X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure |
title_full | X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure |
title_fullStr | X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure |
title_full_unstemmed | X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure |
title_short | X-Ray Phase Nanotomography Resolves the 3D Human Bone Ultrastructure |
title_sort | x-ray phase nanotomography resolves the 3d human bone ultrastructure |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430646/ https://www.ncbi.nlm.nih.gov/pubmed/22952569 http://dx.doi.org/10.1371/journal.pone.0035691 |
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