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Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency
The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence oc...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430704/ https://www.ncbi.nlm.nih.gov/pubmed/22952742 http://dx.doi.org/10.1371/journal.pone.0043708 |
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author | Squirrell, Jayne M. Fong, Jimmy J. Ariza, Carlos A. Mael, Amber Meyer, Kassondra Shevde, Nirupama K. Roopra, Avtar Lyons, Gary E. Kamp, Timothy J. Eliceiri, Kevin W. Ogle, Brenda M. |
author_facet | Squirrell, Jayne M. Fong, Jimmy J. Ariza, Carlos A. Mael, Amber Meyer, Kassondra Shevde, Nirupama K. Roopra, Avtar Lyons, Gary E. Kamp, Timothy J. Eliceiri, Kevin W. Ogle, Brenda M. |
author_sort | Squirrell, Jayne M. |
collection | PubMed |
description | The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications. |
format | Online Article Text |
id | pubmed-3430704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34307042012-09-05 Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency Squirrell, Jayne M. Fong, Jimmy J. Ariza, Carlos A. Mael, Amber Meyer, Kassondra Shevde, Nirupama K. Roopra, Avtar Lyons, Gary E. Kamp, Timothy J. Eliceiri, Kevin W. Ogle, Brenda M. PLoS One Research Article The therapeutic potential of stem cells is limited by the non-uniformity of their phenotypic state. Thus it would be advantageous to noninvasively monitor stem cell status. Driven by this challenge, we employed multidimensional multiphoton microscopy to quantify changes in endogenous fluorescence occurring with pluripotent stem cell differentiation. We found that global and cellular-scale fluorescence lifetime of human embryonic stem cells (hESC) and murine embryonic stem cells (mESC) consistently decreased with differentiation. Less consistent were trends in endogenous fluorescence intensity with differentiation, suggesting intensity is more readily impacted by nuances of species and scale of analysis. What emerges is a practical and accessible approach to evaluate, and ultimately enrich, living stem cell populations based on changes in metabolism that could be exploited for both research and clinical applications. Public Library of Science 2012-08-29 /pmc/articles/PMC3430704/ /pubmed/22952742 http://dx.doi.org/10.1371/journal.pone.0043708 Text en © 2012 Squirrell et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Squirrell, Jayne M. Fong, Jimmy J. Ariza, Carlos A. Mael, Amber Meyer, Kassondra Shevde, Nirupama K. Roopra, Avtar Lyons, Gary E. Kamp, Timothy J. Eliceiri, Kevin W. Ogle, Brenda M. Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency |
title | Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency |
title_full | Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency |
title_fullStr | Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency |
title_full_unstemmed | Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency |
title_short | Endogenous Fluorescence Signatures in Living Pluripotent Stem Cells Change with Loss of Potency |
title_sort | endogenous fluorescence signatures in living pluripotent stem cells change with loss of potency |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430704/ https://www.ncbi.nlm.nih.gov/pubmed/22952742 http://dx.doi.org/10.1371/journal.pone.0043708 |
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