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Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening

The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, and stress responses. However, the precise role of NAC TFs in relation to fruit ripening is poorly understood. In this study, six NAC genes, designated MaNAC1–MaNAC6, were i...

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Autores principales: Shan, Wei, Kuang, Jian-fei, Chen, Lei, Xie, Hui, Peng, Huan-huan, Xiao, Yun-yi, Li, Xue-ping, Chen, Wei-xin, He, Quan-guang, Chen, Jian-ye, Lu, Wang-jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430993/
https://www.ncbi.nlm.nih.gov/pubmed/22888129
http://dx.doi.org/10.1093/jxb/ers178
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author Shan, Wei
Kuang, Jian-fei
Chen, Lei
Xie, Hui
Peng, Huan-huan
Xiao, Yun-yi
Li, Xue-ping
Chen, Wei-xin
He, Quan-guang
Chen, Jian-ye
Lu, Wang-jin
author_facet Shan, Wei
Kuang, Jian-fei
Chen, Lei
Xie, Hui
Peng, Huan-huan
Xiao, Yun-yi
Li, Xue-ping
Chen, Wei-xin
He, Quan-guang
Chen, Jian-ye
Lu, Wang-jin
author_sort Shan, Wei
collection PubMed
description The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, and stress responses. However, the precise role of NAC TFs in relation to fruit ripening is poorly understood. In this study, six NAC genes, designated MaNAC1–MaNAC6, were isolated and characterized from banana fruit. Subcellular localization showed that MaNAC1–MaNAC5 proteins localized preferentially to the nucleus, while MaNAC6 was distributed throughout the entire cell. A transactivation assay in yeast demonstrated that MaNAC4 and MaNAC6, as well as their C-terminal regions, possessed trans-activation activity. Gene expression profiles in fruit with four different ripening characteristics, including natural, ethylene-induced, 1-methylcyclopropene (1-MCP)-delayed, and a combination of 1-MCP with ethylene treatment, revealed that the MaNAC genes were differentially expressed in peel and pulp during post-harvest ripening. MaNAC1 and MaNAC2 were apparently upregulated by ethylene in peel and pulp, consistent with the increase in ethylene production. In contrast, MaNAC3 in peel and pulp and MaNAC5 in peel were constitutively expressed, and transcripts of MaNAC4 in peel and pulp and MaNAC6 in peel decreased, while MaNAC5 or MaNAC6 in pulp increased slightly during fruit ripening. Furthermore, the MaNAC2 promoter was activated after ethylene application, further enhancing the involvement of MaNAC2 in fruit ripening. More importantly, yeast two-hybrid and bimolecular fluorescence complementation analyses confirmed that MaNAC1/2 physically interacted with a downstream component of ethylene signalling, ethylene insensitive 3 (EIN3)-like protein, termed MaEIL5, which was downregulated during ripening. Taken together, these results suggest that MaNACs such as MaNAC1/MaNAC2, may be involved in banana fruit ripening via interaction with ethylene signalling components.
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spelling pubmed-34309932012-08-30 Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening Shan, Wei Kuang, Jian-fei Chen, Lei Xie, Hui Peng, Huan-huan Xiao, Yun-yi Li, Xue-ping Chen, Wei-xin He, Quan-guang Chen, Jian-ye Lu, Wang-jin J Exp Bot Research Paper The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors (TFs) play important roles in plant growth, development, and stress responses. However, the precise role of NAC TFs in relation to fruit ripening is poorly understood. In this study, six NAC genes, designated MaNAC1–MaNAC6, were isolated and characterized from banana fruit. Subcellular localization showed that MaNAC1–MaNAC5 proteins localized preferentially to the nucleus, while MaNAC6 was distributed throughout the entire cell. A transactivation assay in yeast demonstrated that MaNAC4 and MaNAC6, as well as their C-terminal regions, possessed trans-activation activity. Gene expression profiles in fruit with four different ripening characteristics, including natural, ethylene-induced, 1-methylcyclopropene (1-MCP)-delayed, and a combination of 1-MCP with ethylene treatment, revealed that the MaNAC genes were differentially expressed in peel and pulp during post-harvest ripening. MaNAC1 and MaNAC2 were apparently upregulated by ethylene in peel and pulp, consistent with the increase in ethylene production. In contrast, MaNAC3 in peel and pulp and MaNAC5 in peel were constitutively expressed, and transcripts of MaNAC4 in peel and pulp and MaNAC6 in peel decreased, while MaNAC5 or MaNAC6 in pulp increased slightly during fruit ripening. Furthermore, the MaNAC2 promoter was activated after ethylene application, further enhancing the involvement of MaNAC2 in fruit ripening. More importantly, yeast two-hybrid and bimolecular fluorescence complementation analyses confirmed that MaNAC1/2 physically interacted with a downstream component of ethylene signalling, ethylene insensitive 3 (EIN3)-like protein, termed MaEIL5, which was downregulated during ripening. Taken together, these results suggest that MaNACs such as MaNAC1/MaNAC2, may be involved in banana fruit ripening via interaction with ethylene signalling components. Oxford University Press 2012-09 2012-08-29 /pmc/articles/PMC3430993/ /pubmed/22888129 http://dx.doi.org/10.1093/jxb/ers178 Text en © The Author [2012]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Shan, Wei
Kuang, Jian-fei
Chen, Lei
Xie, Hui
Peng, Huan-huan
Xiao, Yun-yi
Li, Xue-ping
Chen, Wei-xin
He, Quan-guang
Chen, Jian-ye
Lu, Wang-jin
Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening
title Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening
title_full Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening
title_fullStr Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening
title_full_unstemmed Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening
title_short Molecular characterization of banana NAC transcription factors and their interactions with ethylene signalling component EIL during fruit ripening
title_sort molecular characterization of banana nac transcription factors and their interactions with ethylene signalling component eil during fruit ripening
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430993/
https://www.ncbi.nlm.nih.gov/pubmed/22888129
http://dx.doi.org/10.1093/jxb/ers178
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