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Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus

An Arabidopsis thaliana mutant, cbd (carotenoid biosynthesis deficient), was recovered from a mutant population based on its yellow cotyledons, yellow-first true leaves, and stunted growth. Seven-day-old seedlings and mature seeds of this mutant had lower chlorophyll and total carotenoids than the w...

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Autores principales: Yu, Bianyun, Gruber, Margaret Y., Khachatourians, George G., Zhou, Rong, Epp, Delwin J., Hegedus, Dwayne D., Parkin, Isobel A. P., Welsch, Ralf, Hannoufa, Abdelali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430994/
https://www.ncbi.nlm.nih.gov/pubmed/22791829
http://dx.doi.org/10.1093/jxb/ers179
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author Yu, Bianyun
Gruber, Margaret Y.
Khachatourians, George G.
Zhou, Rong
Epp, Delwin J.
Hegedus, Dwayne D.
Parkin, Isobel A. P.
Welsch, Ralf
Hannoufa, Abdelali
author_facet Yu, Bianyun
Gruber, Margaret Y.
Khachatourians, George G.
Zhou, Rong
Epp, Delwin J.
Hegedus, Dwayne D.
Parkin, Isobel A. P.
Welsch, Ralf
Hannoufa, Abdelali
author_sort Yu, Bianyun
collection PubMed
description An Arabidopsis thaliana mutant, cbd (carotenoid biosynthesis deficient), was recovered from a mutant population based on its yellow cotyledons, yellow-first true leaves, and stunted growth. Seven-day-old seedlings and mature seeds of this mutant had lower chlorophyll and total carotenoids than the wild type (WT). Genetic and molecular characterization revealed that cbd was a recessive mutant caused by a T-DNA insertion in the gene cpSRP54 encoding the 54kDa subunit of the chloroplast signal recognition particle. Transcript levels of most of the main carotenoid biosynthetic genes in cbd were unchanged relative to WT, but expression increased in carotenoid and abscisic acid catabolic genes. The chloroplasts of cbd also had developmental defects that contributed to decreased carotenoid and chlorophyll contents. Transcription of AtGLK1 (Golden 2-like 1), AtGLK2, and GUN4 appeared to be disrupted in the cbd mutant suggesting that the plastid-to-nucleus retrograde signal may be affected, regulating the changes in chloroplast functional and developmental states and carotenoid content flux. Transformation of A. thaliana and Brassica napus with a gDNA encoding the Arabidopsis cpSRP54 showed the utility of this gene in enhancing levels of seed carotenoids without affecting growth or seed yield.
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spelling pubmed-34309942012-08-30 Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus Yu, Bianyun Gruber, Margaret Y. Khachatourians, George G. Zhou, Rong Epp, Delwin J. Hegedus, Dwayne D. Parkin, Isobel A. P. Welsch, Ralf Hannoufa, Abdelali J Exp Bot Research Paper An Arabidopsis thaliana mutant, cbd (carotenoid biosynthesis deficient), was recovered from a mutant population based on its yellow cotyledons, yellow-first true leaves, and stunted growth. Seven-day-old seedlings and mature seeds of this mutant had lower chlorophyll and total carotenoids than the wild type (WT). Genetic and molecular characterization revealed that cbd was a recessive mutant caused by a T-DNA insertion in the gene cpSRP54 encoding the 54kDa subunit of the chloroplast signal recognition particle. Transcript levels of most of the main carotenoid biosynthetic genes in cbd were unchanged relative to WT, but expression increased in carotenoid and abscisic acid catabolic genes. The chloroplasts of cbd also had developmental defects that contributed to decreased carotenoid and chlorophyll contents. Transcription of AtGLK1 (Golden 2-like 1), AtGLK2, and GUN4 appeared to be disrupted in the cbd mutant suggesting that the plastid-to-nucleus retrograde signal may be affected, regulating the changes in chloroplast functional and developmental states and carotenoid content flux. Transformation of A. thaliana and Brassica napus with a gDNA encoding the Arabidopsis cpSRP54 showed the utility of this gene in enhancing levels of seed carotenoids without affecting growth or seed yield. Oxford University Press 2012-09 2012-07-12 /pmc/articles/PMC3430994/ /pubmed/22791829 http://dx.doi.org/10.1093/jxb/ers179 Text en © 2012 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/3.0/uk/) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Yu, Bianyun
Gruber, Margaret Y.
Khachatourians, George G.
Zhou, Rong
Epp, Delwin J.
Hegedus, Dwayne D.
Parkin, Isobel A. P.
Welsch, Ralf
Hannoufa, Abdelali
Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus
title Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus
title_full Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus
title_fullStr Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus
title_full_unstemmed Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus
title_short Arabidopsis cpSRP54 regulates carotenoid accumulation in Arabidopsis and Brassica napus
title_sort arabidopsis cpsrp54 regulates carotenoid accumulation in arabidopsis and brassica napus
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3430994/
https://www.ncbi.nlm.nih.gov/pubmed/22791829
http://dx.doi.org/10.1093/jxb/ers179
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