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Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs
The surface exposed capsid proteins, VP1, VP2 and VP3, of foot-and-mouth disease virus (FMDV) determine its antigenicity and the ability of the virus to interact with host-cell receptors. Hence, modification of these structural proteins may alter the properties of the virus. In the present study we...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431240/ https://www.ncbi.nlm.nih.gov/pubmed/22624592 http://dx.doi.org/10.1186/1297-9716-43-46 |
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author | Lohse, Louise Jackson, Terry Bøtner, Anette Belsham, Graham J |
author_facet | Lohse, Louise Jackson, Terry Bøtner, Anette Belsham, Graham J |
author_sort | Lohse, Louise |
collection | PubMed |
description | The surface exposed capsid proteins, VP1, VP2 and VP3, of foot-and-mouth disease virus (FMDV) determine its antigenicity and the ability of the virus to interact with host-cell receptors. Hence, modification of these structural proteins may alter the properties of the virus. In the present study we compared the pathogenicity of different FMDVs in young pigs. In total 32 pigs, 7-weeks-old, were exposed to virus, either by direct inoculation or through contact with inoculated pigs, using cell culture adapted (O1K B64), chimeric (O1K/A-TUR and O1K/O-UKG) or field strain (O-UKG/34/2001) viruses. The O1K B64 virus and the two chimeric viruses are identical to each other except for the capsid coding region. Animals exposed to O1K B64 did not exhibit signs of disease, while pigs exposed to each of the other viruses showed typical clinical signs of foot-and-mouth disease (FMD). All pigs infected with the O1K/O-UKG chimera or the field strain (O-UKG/34/2001) developed fulminant disease. Furthermore, 3 of 4 in-contact pigs exposed to the O1K/O-UKG virus died in the acute phase of infection, likely from myocardial infection. However, in the group exposed to the O1K/A-TUR chimeric virus, only 1 pig showed symptoms of disease within the time frame of the experiment (10 days). All pigs that developed clinical disease showed a high level of viral RNA in serum and infected pigs that survived the acute phase of infection developed a serotype specific antibody response. It is concluded that the capsid coding sequences are determinants of FMDV pathogenicity in pigs. |
format | Online Article Text |
id | pubmed-3431240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34312402012-08-31 Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs Lohse, Louise Jackson, Terry Bøtner, Anette Belsham, Graham J Vet Res Research The surface exposed capsid proteins, VP1, VP2 and VP3, of foot-and-mouth disease virus (FMDV) determine its antigenicity and the ability of the virus to interact with host-cell receptors. Hence, modification of these structural proteins may alter the properties of the virus. In the present study we compared the pathogenicity of different FMDVs in young pigs. In total 32 pigs, 7-weeks-old, were exposed to virus, either by direct inoculation or through contact with inoculated pigs, using cell culture adapted (O1K B64), chimeric (O1K/A-TUR and O1K/O-UKG) or field strain (O-UKG/34/2001) viruses. The O1K B64 virus and the two chimeric viruses are identical to each other except for the capsid coding region. Animals exposed to O1K B64 did not exhibit signs of disease, while pigs exposed to each of the other viruses showed typical clinical signs of foot-and-mouth disease (FMD). All pigs infected with the O1K/O-UKG chimera or the field strain (O-UKG/34/2001) developed fulminant disease. Furthermore, 3 of 4 in-contact pigs exposed to the O1K/O-UKG virus died in the acute phase of infection, likely from myocardial infection. However, in the group exposed to the O1K/A-TUR chimeric virus, only 1 pig showed symptoms of disease within the time frame of the experiment (10 days). All pigs that developed clinical disease showed a high level of viral RNA in serum and infected pigs that survived the acute phase of infection developed a serotype specific antibody response. It is concluded that the capsid coding sequences are determinants of FMDV pathogenicity in pigs. BioMed Central 2012 2012-05-24 /pmc/articles/PMC3431240/ /pubmed/22624592 http://dx.doi.org/10.1186/1297-9716-43-46 Text en Copyright ©2012 Lohse et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Lohse, Louise Jackson, Terry Bøtner, Anette Belsham, Graham J Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
title | Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
title_full | Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
title_fullStr | Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
title_full_unstemmed | Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
title_short | Capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
title_sort | capsid coding sequences of foot-and-mouth disease viruses are determinants of pathogenicity in pigs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431240/ https://www.ncbi.nlm.nih.gov/pubmed/22624592 http://dx.doi.org/10.1186/1297-9716-43-46 |
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