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Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure
BACKGROUND: Giardia duodenalis is a common protozoan parasite of humans and animals. Genetic characterization of single loci indicates the existence of eight groups called assemblages, which differ in their host distribution. Molecular analyses challenged the idea that G. duodenalis is a strictly cl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431248/ https://www.ncbi.nlm.nih.gov/pubmed/22882997 http://dx.doi.org/10.1186/1756-3305-5-168 |
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author | Takumi, Katsuhisa Swart, Arno Mank, Theo Lasek-Nesselquist, Erica Lebbad, Marianne Cacciò, Simone M Sprong, Hein |
author_facet | Takumi, Katsuhisa Swart, Arno Mank, Theo Lasek-Nesselquist, Erica Lebbad, Marianne Cacciò, Simone M Sprong, Hein |
author_sort | Takumi, Katsuhisa |
collection | PubMed |
description | BACKGROUND: Giardia duodenalis is a common protozoan parasite of humans and animals. Genetic characterization of single loci indicates the existence of eight groups called assemblages, which differ in their host distribution. Molecular analyses challenged the idea that G. duodenalis is a strictly clonal diplomonad by providing evidence of recombination within and between assemblages. Particularly, inter-assemblage recombination events would complicate the interpretation of multi-locus genotyping data from field isolates: where is a host infected with multiple Giardia genotypes or with a single, recombined Giardia genotype. METHODS: Population genetic analyses on the single and multiple-locus level on an extensive dataset of G. duodenalis isolates from humans and animals were performed. RESULTS: Our analyses indicate that recombination between isolates from different assemblages are apparently very rare or absent in the natural population of Giardia duodenalis. At the multi-locus level, our statistical analyses are more congruent with clonal reproduction and can equally well be explained with the presence of multiple G. duodenalis genotypes within one field isolate. CONCLUSIONS: We conclude that recombination between G. duodenalis assemblages is either very rare or absent. Recombination between genotypes from the same assemblage and genetic exchange between the nuclei of a single cyst needs further investigation. |
format | Online Article Text |
id | pubmed-3431248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34312482012-09-05 Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure Takumi, Katsuhisa Swart, Arno Mank, Theo Lasek-Nesselquist, Erica Lebbad, Marianne Cacciò, Simone M Sprong, Hein Parasit Vectors Research BACKGROUND: Giardia duodenalis is a common protozoan parasite of humans and animals. Genetic characterization of single loci indicates the existence of eight groups called assemblages, which differ in their host distribution. Molecular analyses challenged the idea that G. duodenalis is a strictly clonal diplomonad by providing evidence of recombination within and between assemblages. Particularly, inter-assemblage recombination events would complicate the interpretation of multi-locus genotyping data from field isolates: where is a host infected with multiple Giardia genotypes or with a single, recombined Giardia genotype. METHODS: Population genetic analyses on the single and multiple-locus level on an extensive dataset of G. duodenalis isolates from humans and animals were performed. RESULTS: Our analyses indicate that recombination between isolates from different assemblages are apparently very rare or absent in the natural population of Giardia duodenalis. At the multi-locus level, our statistical analyses are more congruent with clonal reproduction and can equally well be explained with the presence of multiple G. duodenalis genotypes within one field isolate. CONCLUSIONS: We conclude that recombination between G. duodenalis assemblages is either very rare or absent. Recombination between genotypes from the same assemblage and genetic exchange between the nuclei of a single cyst needs further investigation. BioMed Central 2012-08-10 /pmc/articles/PMC3431248/ /pubmed/22882997 http://dx.doi.org/10.1186/1756-3305-5-168 Text en Copyright ©2012 Takumi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Takumi, Katsuhisa Swart, Arno Mank, Theo Lasek-Nesselquist, Erica Lebbad, Marianne Cacciò, Simone M Sprong, Hein Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure |
title | Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure |
title_full | Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure |
title_fullStr | Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure |
title_full_unstemmed | Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure |
title_short | Population-based analyses of Giardia duodenalis is consistent with the clonal assemblage structure |
title_sort | population-based analyses of giardia duodenalis is consistent with the clonal assemblage structure |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431248/ https://www.ncbi.nlm.nih.gov/pubmed/22882997 http://dx.doi.org/10.1186/1756-3305-5-168 |
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