Cargando…

Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs

Effects of probiotic bacteria on viral infections have been described previously. Here, two groups of sows and their piglets were fed with or without feed supplementation of the probiotic bacterium Enterococcus faecium NCIMB 10415. Shedding of enteric viruses naturally occurring in these pigs was an...

Descripción completa

Detalles Bibliográficos
Autores principales: Kreuzer, Susanne, Machnowska, Patrycja, Aßmus, Jens, Sieber, Matthias, Pieper, Robert, Schmidt, Michael FG, Brockmann, Gudrun A, Scharek-Tedin, Lydia, Johne, Reimar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431279/
https://www.ncbi.nlm.nih.gov/pubmed/22838386
http://dx.doi.org/10.1186/1297-9716-43-58
_version_ 1782242054349258752
author Kreuzer, Susanne
Machnowska, Patrycja
Aßmus, Jens
Sieber, Matthias
Pieper, Robert
Schmidt, Michael FG
Brockmann, Gudrun A
Scharek-Tedin, Lydia
Johne, Reimar
author_facet Kreuzer, Susanne
Machnowska, Patrycja
Aßmus, Jens
Sieber, Matthias
Pieper, Robert
Schmidt, Michael FG
Brockmann, Gudrun A
Scharek-Tedin, Lydia
Johne, Reimar
author_sort Kreuzer, Susanne
collection PubMed
description Effects of probiotic bacteria on viral infections have been described previously. Here, two groups of sows and their piglets were fed with or without feed supplementation of the probiotic bacterium Enterococcus faecium NCIMB 10415. Shedding of enteric viruses naturally occurring in these pigs was analyzed by quantitative real-time RT-PCR. No differences between the groups were recorded for hepatitis E virus, encephalomyocarditis virus and norovirus. In contrast, astrovirus was exclusively detected in the non-supplemented control group. Rotavirus was shedded later and with lower amounts in the probiotic piglet group (p < 0.05); rotavirus-shedding piglets gained less weight than non-infected animals (p < 0.05). Serum titres of anti-rotavirus IgA and IgG antibodies were higher in piglets from the control group, whereas no difference was detected between sow groups. Phenotype analysis of immune cell antigens revealed significant differences of the CD4 and CD8β (p < 0.05) as well as CD8α and CD25 (p < 0.1) T cell populations of the probiotic supplemented group compared to the non-supplemented control group. In addition, differences were evident for CD21/MHCII-positive (p < 0.05) and IgM-positive (p < 0.1) B cell populations. The results indicate that probiotic bacteria could have effects on virus shedding in naturally infected pigs, which depend on the virus type. These effects seem to be caused by immunological changes; however, the distinct mechanism of action remains to be elucidated.
format Online
Article
Text
id pubmed-3431279
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-34312792012-08-31 Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs Kreuzer, Susanne Machnowska, Patrycja Aßmus, Jens Sieber, Matthias Pieper, Robert Schmidt, Michael FG Brockmann, Gudrun A Scharek-Tedin, Lydia Johne, Reimar Vet Res Research Effects of probiotic bacteria on viral infections have been described previously. Here, two groups of sows and their piglets were fed with or without feed supplementation of the probiotic bacterium Enterococcus faecium NCIMB 10415. Shedding of enteric viruses naturally occurring in these pigs was analyzed by quantitative real-time RT-PCR. No differences between the groups were recorded for hepatitis E virus, encephalomyocarditis virus and norovirus. In contrast, astrovirus was exclusively detected in the non-supplemented control group. Rotavirus was shedded later and with lower amounts in the probiotic piglet group (p < 0.05); rotavirus-shedding piglets gained less weight than non-infected animals (p < 0.05). Serum titres of anti-rotavirus IgA and IgG antibodies were higher in piglets from the control group, whereas no difference was detected between sow groups. Phenotype analysis of immune cell antigens revealed significant differences of the CD4 and CD8β (p < 0.05) as well as CD8α and CD25 (p < 0.1) T cell populations of the probiotic supplemented group compared to the non-supplemented control group. In addition, differences were evident for CD21/MHCII-positive (p < 0.05) and IgM-positive (p < 0.1) B cell populations. The results indicate that probiotic bacteria could have effects on virus shedding in naturally infected pigs, which depend on the virus type. These effects seem to be caused by immunological changes; however, the distinct mechanism of action remains to be elucidated. BioMed Central 2012 2012-07-27 /pmc/articles/PMC3431279/ /pubmed/22838386 http://dx.doi.org/10.1186/1297-9716-43-58 Text en Copyright ©2012 Kreuzer et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Kreuzer, Susanne
Machnowska, Patrycja
Aßmus, Jens
Sieber, Matthias
Pieper, Robert
Schmidt, Michael FG
Brockmann, Gudrun A
Scharek-Tedin, Lydia
Johne, Reimar
Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs
title Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs
title_full Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs
title_fullStr Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs
title_full_unstemmed Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs
title_short Feeding of the probiotic bacterium Enterococcus faecium NCIMB 10415 differentially affects shedding of enteric viruses in pigs
title_sort feeding of the probiotic bacterium enterococcus faecium ncimb 10415 differentially affects shedding of enteric viruses in pigs
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431279/
https://www.ncbi.nlm.nih.gov/pubmed/22838386
http://dx.doi.org/10.1186/1297-9716-43-58
work_keys_str_mv AT kreuzersusanne feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT machnowskapatrycja feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT aßmusjens feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT siebermatthias feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT pieperrobert feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT schmidtmichaelfg feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT brockmanngudruna feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT scharektedinlydia feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs
AT johnereimar feedingoftheprobioticbacteriumenterococcusfaeciumncimb10415differentiallyaffectssheddingofentericvirusesinpigs