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Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia

BACKGROUND: Osteoprotegerin (OPG), a key regulatory factor in bone metabolism, was documented also a potential pro-angiogenic factor, which acts an important role in protecting vascular endothelial cells. Since preeclampsia has gradually been employed to be vascular diseases, we speculated that OPG...

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Autores principales: Shen, Pei, Gong, Yunhui, Wang, Tao, Chen, Yueyue, Jia, Jin, Ni, Shanshan, Zhou, Bin, Song, Yapin, Zhang, Lin, Zhou, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431377/
https://www.ncbi.nlm.nih.gov/pubmed/22952959
http://dx.doi.org/10.1371/journal.pone.0044340
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author Shen, Pei
Gong, Yunhui
Wang, Tao
Chen, Yueyue
Jia, Jin
Ni, Shanshan
Zhou, Bin
Song, Yapin
Zhang, Lin
Zhou, Rong
author_facet Shen, Pei
Gong, Yunhui
Wang, Tao
Chen, Yueyue
Jia, Jin
Ni, Shanshan
Zhou, Bin
Song, Yapin
Zhang, Lin
Zhou, Rong
author_sort Shen, Pei
collection PubMed
description BACKGROUND: Osteoprotegerin (OPG), a key regulatory factor in bone metabolism, was documented also a potential pro-angiogenic factor, which acts an important role in protecting vascular endothelial cells. Since preeclampsia has gradually been employed to be vascular diseases, we speculated that OPG might be associated with preeclampsia. The study was to evaluate the level of OPG protein and mRNA in placenta, and investigate the relationship between OPG and the pathogenesis of preeclampsia. METHODOLOGY/PRINCIPAL FINDINGS: Placental specimens from 30 term normal pregnancy, 30 severe preeclampsia and 30 mild cases were studied. The expression and levels of OPGs’ protein and mRNA were detected by immunohistochemisty, western blot analysis and real-time quantitative PCR analysis respectively. The expression of OPG protein was found in cytoplasm of placenta cytotrophoblasts and syncytiotrophoblasts in three groups. There were no significant differences of OPG protein between the maternal and fetal side in each group. The OPG protein and mRNA levels in severe preeclampsia were significantly higher than those in mild cases and normal pregnancy. However, there were no markedly differences of the OPG protein and mRNA levels between term delivery and preterm delivery in severe cases. In preeclampsia, the OPG protein and mRNA level was positively correlated with systolic blood pressure and 24 h urinary protein respectively. CONCLUSIONS/SIGNIFICANCE: OPG protein and mRNA level in placentas of preeclampsia were found abnormal compared with normal pregnancy. In preeclampsia, the OPG protein and mRNA levels were closely related with its important clinical parameters. Taken together, OPG might be closely correlated with the pathogenesis of preeclampsia.
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spelling pubmed-34313772012-09-05 Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia Shen, Pei Gong, Yunhui Wang, Tao Chen, Yueyue Jia, Jin Ni, Shanshan Zhou, Bin Song, Yapin Zhang, Lin Zhou, Rong PLoS One Research Article BACKGROUND: Osteoprotegerin (OPG), a key regulatory factor in bone metabolism, was documented also a potential pro-angiogenic factor, which acts an important role in protecting vascular endothelial cells. Since preeclampsia has gradually been employed to be vascular diseases, we speculated that OPG might be associated with preeclampsia. The study was to evaluate the level of OPG protein and mRNA in placenta, and investigate the relationship between OPG and the pathogenesis of preeclampsia. METHODOLOGY/PRINCIPAL FINDINGS: Placental specimens from 30 term normal pregnancy, 30 severe preeclampsia and 30 mild cases were studied. The expression and levels of OPGs’ protein and mRNA were detected by immunohistochemisty, western blot analysis and real-time quantitative PCR analysis respectively. The expression of OPG protein was found in cytoplasm of placenta cytotrophoblasts and syncytiotrophoblasts in three groups. There were no significant differences of OPG protein between the maternal and fetal side in each group. The OPG protein and mRNA levels in severe preeclampsia were significantly higher than those in mild cases and normal pregnancy. However, there were no markedly differences of the OPG protein and mRNA levels between term delivery and preterm delivery in severe cases. In preeclampsia, the OPG protein and mRNA level was positively correlated with systolic blood pressure and 24 h urinary protein respectively. CONCLUSIONS/SIGNIFICANCE: OPG protein and mRNA level in placentas of preeclampsia were found abnormal compared with normal pregnancy. In preeclampsia, the OPG protein and mRNA levels were closely related with its important clinical parameters. Taken together, OPG might be closely correlated with the pathogenesis of preeclampsia. Public Library of Science 2012-08-30 /pmc/articles/PMC3431377/ /pubmed/22952959 http://dx.doi.org/10.1371/journal.pone.0044340 Text en © 2012 Shen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Shen, Pei
Gong, Yunhui
Wang, Tao
Chen, Yueyue
Jia, Jin
Ni, Shanshan
Zhou, Bin
Song, Yapin
Zhang, Lin
Zhou, Rong
Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia
title Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia
title_full Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia
title_fullStr Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia
title_full_unstemmed Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia
title_short Expression of Osteoprotegerin in Placenta and Its Association with Preeclampsia
title_sort expression of osteoprotegerin in placenta and its association with preeclampsia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431377/
https://www.ncbi.nlm.nih.gov/pubmed/22952959
http://dx.doi.org/10.1371/journal.pone.0044340
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