Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy

We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before clo...

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Detalles Bibliográficos
Autores principales: Kawashima, Rei, Kawamura, Yuki I., Oshio, Tomoyuki, Mizutani, Noriko, Okada, Toshihiko, Kawamura, Yutaka J., Konishi, Fumio, Dohi, Taeko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431549/
https://www.ncbi.nlm.nih.gov/pubmed/22738652
http://dx.doi.org/10.1080/15321819.2011.638409
Descripción
Sumario:We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans.

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