Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy

We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before clo...

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Autores principales: Kawashima, Rei, Kawamura, Yuki I., Oshio, Tomoyuki, Mizutani, Noriko, Okada, Toshihiko, Kawamura, Yutaka J., Konishi, Fumio, Dohi, Taeko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431549/
https://www.ncbi.nlm.nih.gov/pubmed/22738652
http://dx.doi.org/10.1080/15321819.2011.638409
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author Kawashima, Rei
Kawamura, Yuki I.
Oshio, Tomoyuki
Mizutani, Noriko
Okada, Toshihiko
Kawamura, Yutaka J.
Konishi, Fumio
Dohi, Taeko
author_facet Kawashima, Rei
Kawamura, Yuki I.
Oshio, Tomoyuki
Mizutani, Noriko
Okada, Toshihiko
Kawamura, Yutaka J.
Konishi, Fumio
Dohi, Taeko
author_sort Kawashima, Rei
collection PubMed
description We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans.
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spelling pubmed-34315492012-08-31 Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy Kawashima, Rei Kawamura, Yuki I. Oshio, Tomoyuki Mizutani, Noriko Okada, Toshihiko Kawamura, Yutaka J. Konishi, Fumio Dohi, Taeko J Immunoassay Immunochem Research Article We recently found that chemokine-driven peritoneal cell aggregation is the primary mechanism of postoperative adhesion in a mouse model. To investigate this in humans, paired samples of peritoneal lavage fluid were obtained from seven patients immediately after incision (preoperative) and before closure (postoperative), and were assayed for the presence of 27 cytokines and chemokines using multiplex beads assay. As a result, IL-6 and CCL5 showed the most striking increase during operation. Recombinant CCL5 or lavage fluid induced chemotaxis of human peripheral blood mononuclear cells. We propose that CCL5 is possibly involved in the mechanism of postoperative adhesion in humans. Taylor & Francis 2012-06-27 2012-07 /pmc/articles/PMC3431549/ /pubmed/22738652 http://dx.doi.org/10.1080/15321819.2011.638409 Text en Copyright © Taylor & Francis Group, LLC http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf This is an open access article distributed under the Supplemental Terms and Conditions for iOpenAccess articles published in Taylor & Francis journals (http://www.informaworld.com/mpp/uploads/iopenaccess_tcs.pdf) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kawashima, Rei
Kawamura, Yuki I.
Oshio, Tomoyuki
Mizutani, Noriko
Okada, Toshihiko
Kawamura, Yutaka J.
Konishi, Fumio
Dohi, Taeko
Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_full Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_fullStr Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_full_unstemmed Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_short Comprehensive Analysis of Chemokines and Cytokines Secreted in the Peritoneal Cavity During Laparotomy
title_sort comprehensive analysis of chemokines and cytokines secreted in the peritoneal cavity during laparotomy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431549/
https://www.ncbi.nlm.nih.gov/pubmed/22738652
http://dx.doi.org/10.1080/15321819.2011.638409
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