Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)

Brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Pol...

Descripción completa

Detalles Bibliográficos
Autores principales: Jing, Shengli, Zhou, Xi, Yu, Hangjin, Liu, Bingfang, Zhang, Chunxiao, Wang, Shuzhen, Peng, Xinxin, Zhu, Lili, Ding, Yi, He, Guangcun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Short Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431811/
https://www.ncbi.nlm.nih.gov/pubmed/22949813
http://dx.doi.org/10.3390/ijms13089527
_version_ 1782242106628112384
author Jing, Shengli
Zhou, Xi
Yu, Hangjin
Liu, Bingfang
Zhang, Chunxiao
Wang, Shuzhen
Peng, Xinxin
Zhu, Lili
Ding, Yi
He, Guangcun
author_facet Jing, Shengli
Zhou, Xi
Yu, Hangjin
Liu, Bingfang
Zhang, Chunxiao
Wang, Shuzhen
Peng, Xinxin
Zhu, Lili
Ding, Yi
He, Guangcun
author_sort Jing, Shengli
collection PubMed
description Brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics.
format Online
Article
Text
id pubmed-3431811
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Molecular Diversity Preservation International (MDPI)
record_format MEDLINE/PubMed
spelling pubmed-34318112012-09-04 Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål) Jing, Shengli Zhou, Xi Yu, Hangjin Liu, Bingfang Zhang, Chunxiao Wang, Shuzhen Peng, Xinxin Zhu, Lili Ding, Yi He, Guangcun Int J Mol Sci Short Note Brown planthopper (Nilaparvata lugens Stål) (Homoptera: Delphacidae) is an economically important pest on rice. In this study, 30 polymorphic microsatellite markers were developed from N. lugens genomic libraries using the method of Fast Isolation by AFLP of Sequence Containing Repeats (FIASCO). Polymorphism of each locus was detected in 48 individuals from two natural populations. These microsatellite loci revealed 2 to 18 alleles, and the expected and observed heterozygosities ranged from 0.042 to 0.937 and from 0.042 to 0.958, respectively. These markers will be useful for the future study of this agricultural pest in population genetics and molecular genetics. Molecular Diversity Preservation International (MDPI) 2012-07-27 /pmc/articles/PMC3431811/ /pubmed/22949813 http://dx.doi.org/10.3390/ijms13089527 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Short Note
Jing, Shengli
Zhou, Xi
Yu, Hangjin
Liu, Bingfang
Zhang, Chunxiao
Wang, Shuzhen
Peng, Xinxin
Zhu, Lili
Ding, Yi
He, Guangcun
Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)
title Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)
title_full Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)
title_fullStr Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)
title_full_unstemmed Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)
title_short Isolation and Characterization of Microsatellite Markers in Brown Planthopper (Nilaparvata lugens Stål)
title_sort isolation and characterization of microsatellite markers in brown planthopper (nilaparvata lugens stål)
topic Short Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431811/
https://www.ncbi.nlm.nih.gov/pubmed/22949813
http://dx.doi.org/10.3390/ijms13089527
work_keys_str_mv AT jingshengli isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT zhouxi isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT yuhangjin isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT liubingfang isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT zhangchunxiao isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT wangshuzhen isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT pengxinxin isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT zhulili isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT dingyi isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal
AT heguangcun isolationandcharacterizationofmicrosatellitemarkersinbrownplanthoppernilaparvatalugensstal

Ejemplares similares