Cargando…
An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids
The mitochondrial outer membrane protein Tom40 is the general entry gate for imported proteins in essentially all eukaryotes. Trypanosomatids lack Tom40, however, and use instead a protein termed the archaic translocase of the outer mitochondrial membrane (ATOM). Here we report the discovery of pATO...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431924/ https://www.ncbi.nlm.nih.gov/pubmed/22787278 http://dx.doi.org/10.1091/mbc.E12-02-0107 |
_version_ | 1782242129156767744 |
---|---|
author | Pusnik, Mascha Mani, Jan Schmidt, Oliver Niemann, Moritz Oeljeklaus, Silke Schnarwiler, Felix Warscheid, Bettina Lithgow, Trevor Meisinger, Chris Schneider, André |
author_facet | Pusnik, Mascha Mani, Jan Schmidt, Oliver Niemann, Moritz Oeljeklaus, Silke Schnarwiler, Felix Warscheid, Bettina Lithgow, Trevor Meisinger, Chris Schneider, André |
author_sort | Pusnik, Mascha |
collection | PubMed |
description | The mitochondrial outer membrane protein Tom40 is the general entry gate for imported proteins in essentially all eukaryotes. Trypanosomatids lack Tom40, however, and use instead a protein termed the archaic translocase of the outer mitochondrial membrane (ATOM). Here we report the discovery of pATOM36, a novel essential component of the trypanosomal outer membrane protein import system that interacts with ATOM. pATOM36 is not related to known Tom proteins from other organisms and mediates the import of matrix proteins. However, there is a group of precursor proteins whose import is independent of pATOM36. Domain-swapping experiments indicate that the N-terminal presequence-containing domain of the substrate proteins at least in part determines the dependence on pATOM36. Secondary structure profiling suggests that pATOM36 is composed largely of α-helices and its assembly into the outer membrane is independent of the sorting and assembly machinery complex. Taken together, these results show that pATOM36 is a novel component associated with the ATOM complex that promotes the import of a subpopulation of proteins into the mitochondrial matrix. |
format | Online Article Text |
id | pubmed-3431924 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-34319242012-11-16 An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids Pusnik, Mascha Mani, Jan Schmidt, Oliver Niemann, Moritz Oeljeklaus, Silke Schnarwiler, Felix Warscheid, Bettina Lithgow, Trevor Meisinger, Chris Schneider, André Mol Biol Cell Articles The mitochondrial outer membrane protein Tom40 is the general entry gate for imported proteins in essentially all eukaryotes. Trypanosomatids lack Tom40, however, and use instead a protein termed the archaic translocase of the outer mitochondrial membrane (ATOM). Here we report the discovery of pATOM36, a novel essential component of the trypanosomal outer membrane protein import system that interacts with ATOM. pATOM36 is not related to known Tom proteins from other organisms and mediates the import of matrix proteins. However, there is a group of precursor proteins whose import is independent of pATOM36. Domain-swapping experiments indicate that the N-terminal presequence-containing domain of the substrate proteins at least in part determines the dependence on pATOM36. Secondary structure profiling suggests that pATOM36 is composed largely of α-helices and its assembly into the outer membrane is independent of the sorting and assembly machinery complex. Taken together, these results show that pATOM36 is a novel component associated with the ATOM complex that promotes the import of a subpopulation of proteins into the mitochondrial matrix. The American Society for Cell Biology 2012-09-01 /pmc/articles/PMC3431924/ /pubmed/22787278 http://dx.doi.org/10.1091/mbc.E12-02-0107 Text en © 2012 Pusnik et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Pusnik, Mascha Mani, Jan Schmidt, Oliver Niemann, Moritz Oeljeklaus, Silke Schnarwiler, Felix Warscheid, Bettina Lithgow, Trevor Meisinger, Chris Schneider, André An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
title | An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
title_full | An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
title_fullStr | An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
title_full_unstemmed | An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
title_short | An essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
title_sort | essential novel component of the noncanonical mitochondrial outer membrane protein import system of trypanosomatids |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3431924/ https://www.ncbi.nlm.nih.gov/pubmed/22787278 http://dx.doi.org/10.1091/mbc.E12-02-0107 |
work_keys_str_mv | AT pusnikmascha anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT manijan anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT schmidtoliver anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT niemannmoritz anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT oeljeklaussilke anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT schnarwilerfelix anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT warscheidbettina anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT lithgowtrevor anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT meisingerchris anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT schneiderandre anessentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT pusnikmascha essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT manijan essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT schmidtoliver essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT niemannmoritz essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT oeljeklaussilke essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT schnarwilerfelix essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT warscheidbettina essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT lithgowtrevor essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT meisingerchris essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids AT schneiderandre essentialnovelcomponentofthenoncanonicalmitochondrialoutermembraneproteinimportsystemoftrypanosomatids |