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Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa
Retinal photoreceptors die during retinal synaptogenesis in a portion of retinal degeneration. Whether cone bipolar cells establish regular retinal mosaics and mature morphologies, and resist degeneration are not completely understood. To explore these issues, we backcrossed a transgenic mouse expre...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432094/ https://www.ncbi.nlm.nih.gov/pubmed/22952865 http://dx.doi.org/10.1371/journal.pone.0044036 |
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author | Chen, Miao Wang, Ke Lin, Bin |
author_facet | Chen, Miao Wang, Ke Lin, Bin |
author_sort | Chen, Miao |
collection | PubMed |
description | Retinal photoreceptors die during retinal synaptogenesis in a portion of retinal degeneration. Whether cone bipolar cells establish regular retinal mosaics and mature morphologies, and resist degeneration are not completely understood. To explore these issues, we backcrossed a transgenic mouse expressing enhanced green fluorescent protein (EGFP) in one subset of cone bipolar cells (type 7) into rd1 mice, a classic mouse model of retinal degeneration, to examine the development and survival of cone bipolar cells in a background of retinal degeneration. Our data revealed that both the development and degeneration of cone bipolar cells are independent of the normal activity of cone photoreceptors. We found that type 7 cone bipolar cells achieved a uniform tiling of the retinal surface and developed normal dendritic and axonal arbors without the influence of cone photoreceptor innervation. On the other hand, degeneration of type 7 cone bipolar cells, contrary to our belief of central-to-peripheral progression, was spatially uniform across the retina independent of the spatiotemporal pattern of cone degeneration. The results have important implications for the design of more effective therapies to restore vision in retinal degeneration. |
format | Online Article Text |
id | pubmed-3432094 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34320942012-09-05 Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa Chen, Miao Wang, Ke Lin, Bin PLoS One Research Article Retinal photoreceptors die during retinal synaptogenesis in a portion of retinal degeneration. Whether cone bipolar cells establish regular retinal mosaics and mature morphologies, and resist degeneration are not completely understood. To explore these issues, we backcrossed a transgenic mouse expressing enhanced green fluorescent protein (EGFP) in one subset of cone bipolar cells (type 7) into rd1 mice, a classic mouse model of retinal degeneration, to examine the development and survival of cone bipolar cells in a background of retinal degeneration. Our data revealed that both the development and degeneration of cone bipolar cells are independent of the normal activity of cone photoreceptors. We found that type 7 cone bipolar cells achieved a uniform tiling of the retinal surface and developed normal dendritic and axonal arbors without the influence of cone photoreceptor innervation. On the other hand, degeneration of type 7 cone bipolar cells, contrary to our belief of central-to-peripheral progression, was spatially uniform across the retina independent of the spatiotemporal pattern of cone degeneration. The results have important implications for the design of more effective therapies to restore vision in retinal degeneration. Public Library of Science 2012-08-31 /pmc/articles/PMC3432094/ /pubmed/22952865 http://dx.doi.org/10.1371/journal.pone.0044036 Text en © 2012 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Miao Wang, Ke Lin, Bin Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa |
title | Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa |
title_full | Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa |
title_fullStr | Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa |
title_full_unstemmed | Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa |
title_short | Development and Degeneration of Cone Bipolar Cells Are Independent of Cone Photoreceptors in a Mouse Model of Retinitis Pigmentosa |
title_sort | development and degeneration of cone bipolar cells are independent of cone photoreceptors in a mouse model of retinitis pigmentosa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432094/ https://www.ncbi.nlm.nih.gov/pubmed/22952865 http://dx.doi.org/10.1371/journal.pone.0044036 |
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