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A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum

BACKGROUND: Schistosomiasis japonica remains a real threat to public health in China. The currently used immunodiagnostic assays are sensitive and have a certain degree of specificity, however, they all use complex crude antigens, are based on detection of schistosome-specific antibodies, and have b...

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Autores principales: Zhu, Yinchang, Hua, Wanquan, Xu, Ming, He, Wei, Wang, Xiaoting, Dai, Yang, Zhao, Song, Tang, Jianxia, Wang, Shixia, Lu, Shan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432135/
https://www.ncbi.nlm.nih.gov/pubmed/22952862
http://dx.doi.org/10.1371/journal.pone.0044032
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author Zhu, Yinchang
Hua, Wanquan
Xu, Ming
He, Wei
Wang, Xiaoting
Dai, Yang
Zhao, Song
Tang, Jianxia
Wang, Shixia
Lu, Shan
author_facet Zhu, Yinchang
Hua, Wanquan
Xu, Ming
He, Wei
Wang, Xiaoting
Dai, Yang
Zhao, Song
Tang, Jianxia
Wang, Shixia
Lu, Shan
author_sort Zhu, Yinchang
collection PubMed
description BACKGROUND: Schistosomiasis japonica remains a real threat to public health in China. The currently used immunodiagnostic assays are sensitive and have a certain degree of specificity, however, they all use complex crude antigens, are based on detection of schistosome-specific antibodies, and have been shown to cross-react with other parasitic diseases. Therefore, these assays cannot be used to evaluate chemotherapy efficacy. The development of highly sensitive and highly specific immunodiagnostic techniques that can monitor the decline of antibodies specific for S. japonica will be extremely valuable as part of the ongoing strategy to control schistosomiasis in endemic areas. Here we report on the identification of unique fraction antigens of soluble egg antigen (SEA) to which the antibodies disappear 7 weeks after effective treatment. Furthermore, we use these SEA fractions to develop a modified assay with both high sensitivity and specificity. METHODOLOGY/PRINCIPAL FINDINGS: SEA of S. japonicum was fractionated by electrophoresis using 7.5% SDS-PAGE under non-reducing conditions. The SEA fraction antigens to which antibodies were decreased soon after treatment were collected and used as the detection antigens to establish the FA-ELISA. Sera from patients with acute and chronic schistosomiasis infection, healthy people, and those with other parasitic diseases, were used to evaluate their sensitivity and specificity. Furthermore, sera from patients with chronic schistosomiasis infection were evaluated before and after treatment at different time points to evaluate their chemotherapeutic efficacy. CONCLUSION/SIGNIFICANCE: We demonstrated that this novel FA-ELISA provided high sensitivity and specificity, with very low cross-reactivity, and can serve as an effective tool to determine the efficacy of chemotherapy against S. japonicum.
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spelling pubmed-34321352012-09-05 A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum Zhu, Yinchang Hua, Wanquan Xu, Ming He, Wei Wang, Xiaoting Dai, Yang Zhao, Song Tang, Jianxia Wang, Shixia Lu, Shan PLoS One Research Article BACKGROUND: Schistosomiasis japonica remains a real threat to public health in China. The currently used immunodiagnostic assays are sensitive and have a certain degree of specificity, however, they all use complex crude antigens, are based on detection of schistosome-specific antibodies, and have been shown to cross-react with other parasitic diseases. Therefore, these assays cannot be used to evaluate chemotherapy efficacy. The development of highly sensitive and highly specific immunodiagnostic techniques that can monitor the decline of antibodies specific for S. japonica will be extremely valuable as part of the ongoing strategy to control schistosomiasis in endemic areas. Here we report on the identification of unique fraction antigens of soluble egg antigen (SEA) to which the antibodies disappear 7 weeks after effective treatment. Furthermore, we use these SEA fractions to develop a modified assay with both high sensitivity and specificity. METHODOLOGY/PRINCIPAL FINDINGS: SEA of S. japonicum was fractionated by electrophoresis using 7.5% SDS-PAGE under non-reducing conditions. The SEA fraction antigens to which antibodies were decreased soon after treatment were collected and used as the detection antigens to establish the FA-ELISA. Sera from patients with acute and chronic schistosomiasis infection, healthy people, and those with other parasitic diseases, were used to evaluate their sensitivity and specificity. Furthermore, sera from patients with chronic schistosomiasis infection were evaluated before and after treatment at different time points to evaluate their chemotherapeutic efficacy. CONCLUSION/SIGNIFICANCE: We demonstrated that this novel FA-ELISA provided high sensitivity and specificity, with very low cross-reactivity, and can serve as an effective tool to determine the efficacy of chemotherapy against S. japonicum. Public Library of Science 2012-08-31 /pmc/articles/PMC3432135/ /pubmed/22952862 http://dx.doi.org/10.1371/journal.pone.0044032 Text en © 2012 Zhu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhu, Yinchang
Hua, Wanquan
Xu, Ming
He, Wei
Wang, Xiaoting
Dai, Yang
Zhao, Song
Tang, Jianxia
Wang, Shixia
Lu, Shan
A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum
title A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum
title_full A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum
title_fullStr A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum
title_full_unstemmed A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum
title_short A Novel Immunodiagnostic Assay to Detect Serum Antibody Response against Selected Soluble Egg Antigen Fractions from Schistosoma japonicum
title_sort novel immunodiagnostic assay to detect serum antibody response against selected soluble egg antigen fractions from schistosoma japonicum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432135/
https://www.ncbi.nlm.nih.gov/pubmed/22952862
http://dx.doi.org/10.1371/journal.pone.0044032
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