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Insertion of a myc-tag within α-dystroglycan domains improves its biochemical and microscopic detection

BACKGROUND: Epitope tags and fluorescent fusion proteins have become indispensable molecular tools for studies in the fields of biochemistry and cell biology. The knowledge collected on the subdomain organization of the two subunits of the adhesion complex dystroglycan (DG) enabled us to insert the...

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Detalles Bibliográficos
Autores principales: Morlacchi, Simona, Sciandra, Francesca, Bigotti, Maria Giulia, Bozzi, Manuela, Hübner, Wolfgang, Galtieri, Antonio, Giardina, Bruno, Brancaccio, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432625/
https://www.ncbi.nlm.nih.gov/pubmed/22835149
http://dx.doi.org/10.1186/1471-2091-13-14