Mouse ooplasm confers context-specific reprogramming capacity

Enucleated oocytes have the remarkable ability to reprogram somatic nuclei back to totipotency. Here we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfe...

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Detalles Bibliográficos
Autores principales: Chan, Michelle M., Smith, Zachary D., Egli, Dieter, Regev, Aviv, Meissner, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432711/
https://www.ncbi.nlm.nih.gov/pubmed/22902786
http://dx.doi.org/10.1038/ng.2382
Descripción
Sumario:Enucleated oocytes have the remarkable ability to reprogram somatic nuclei back to totipotency. Here we investigate genome-scale DNA methylation patterns after nuclear transfer and compare them to the dynamics at fertilization. We identify specific targets for DNA demethylation after nuclear transfer such as germ-line associated promoters, as well as unique limitations that include certain repetitive element classes.

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