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Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1
Embryo morphogenesis is driven by dynamic cell behaviors, including migration, that are coordinated with fate specification and differentiation, but how such coordination is achieved remains poorly understood. During zebrafish gastrulation, endodermal cells sequentially exhibit first random, nonpers...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432772/ https://www.ncbi.nlm.nih.gov/pubmed/22945937 http://dx.doi.org/10.1083/jcb.201203012 |
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author | Woo, Stephanie Housley, Michael P. Weiner, Orion D. Stainier, Didier Y.R. |
author_facet | Woo, Stephanie Housley, Michael P. Weiner, Orion D. Stainier, Didier Y.R. |
author_sort | Woo, Stephanie |
collection | PubMed |
description | Embryo morphogenesis is driven by dynamic cell behaviors, including migration, that are coordinated with fate specification and differentiation, but how such coordination is achieved remains poorly understood. During zebrafish gastrulation, endodermal cells sequentially exhibit first random, nonpersistent migration followed by oriented, persistent migration and finally collective migration. Using a novel transgenic line that labels the endodermal actin cytoskeleton, we found that these stage-dependent changes in migratory behavior correlated with changes in actin dynamics. The dynamic actin and random motility exhibited during early gastrulation were dependent on both Nodal and Rac1 signaling. We further identified the Rac-specific guanine nucleotide exchange factor Prex1 as a Nodal target and showed that it mediated Nodal-dependent random motility. Reducing Rac1 activity in endodermal cells caused them to bypass the random migration phase and aberrantly contribute to mesodermal tissues. Together, our results reveal a novel role for Nodal signaling in regulating actin dynamics and migration behavior, which are crucial for endodermal morphogenesis and cell fate decisions. |
format | Online Article Text |
id | pubmed-3432772 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34327722013-03-03 Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 Woo, Stephanie Housley, Michael P. Weiner, Orion D. Stainier, Didier Y.R. J Cell Biol Research Articles Embryo morphogenesis is driven by dynamic cell behaviors, including migration, that are coordinated with fate specification and differentiation, but how such coordination is achieved remains poorly understood. During zebrafish gastrulation, endodermal cells sequentially exhibit first random, nonpersistent migration followed by oriented, persistent migration and finally collective migration. Using a novel transgenic line that labels the endodermal actin cytoskeleton, we found that these stage-dependent changes in migratory behavior correlated with changes in actin dynamics. The dynamic actin and random motility exhibited during early gastrulation were dependent on both Nodal and Rac1 signaling. We further identified the Rac-specific guanine nucleotide exchange factor Prex1 as a Nodal target and showed that it mediated Nodal-dependent random motility. Reducing Rac1 activity in endodermal cells caused them to bypass the random migration phase and aberrantly contribute to mesodermal tissues. Together, our results reveal a novel role for Nodal signaling in regulating actin dynamics and migration behavior, which are crucial for endodermal morphogenesis and cell fate decisions. The Rockefeller University Press 2012-09-03 /pmc/articles/PMC3432772/ /pubmed/22945937 http://dx.doi.org/10.1083/jcb.201203012 Text en © 2012 Woo et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Woo, Stephanie Housley, Michael P. Weiner, Orion D. Stainier, Didier Y.R. Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 |
title | Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 |
title_full | Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 |
title_fullStr | Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 |
title_full_unstemmed | Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 |
title_short | Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1 |
title_sort | nodal signaling regulates endodermal cell motility and actin dynamics via rac1 and prex1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3432772/ https://www.ncbi.nlm.nih.gov/pubmed/22945937 http://dx.doi.org/10.1083/jcb.201203012 |
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