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The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis
Experimental autoimmune uveitis (EAU) is a well established model for immune-mediated organ-specific disease. Our group has recently shown that the M. leprae Hsp65 aggravated the uveitis in mice; in the present study, we evaluated the action of M. leprae K(409)A mutant protein and the synthetic pep...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433114/ https://www.ncbi.nlm.nih.gov/pubmed/22966424 http://dx.doi.org/10.1155/2012/197648 |
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author | Commodaro, Alessandra Gonçalves Marengo, Eliana Blini Peron, Jean Pierre S. Brandao, Wesley Arslanian, Christina Melo, Robson Lopes Baldon, Estevam J. Belfort, Rubens Sant'Anna, Osvaldo Augusto Rizzo, Luiz Vicente |
author_facet | Commodaro, Alessandra Gonçalves Marengo, Eliana Blini Peron, Jean Pierre S. Brandao, Wesley Arslanian, Christina Melo, Robson Lopes Baldon, Estevam J. Belfort, Rubens Sant'Anna, Osvaldo Augusto Rizzo, Luiz Vicente |
author_sort | Commodaro, Alessandra Gonçalves |
collection | PubMed |
description | Experimental autoimmune uveitis (EAU) is a well established model for immune-mediated organ-specific disease. Our group has recently shown that the M. leprae Hsp65 aggravated the uveitis in mice; in the present study, we evaluated the action of M. leprae K(409)A mutant protein and the synthetic peptides Leader pep and K(409)A pep (covering amino acids residues 352–371 of WT and K(409)A proteins of M. leprae Hsp65, resp.) on the pathogenesis of EAU. Mice received the 161–180 IRBP peptide and B. pertussis toxin followed by the intraperitoneal inoculation of K(409)A protein or the Leader pep or K(409)A pep. The Leader pep aggravated the disease, but mice receiving the K(409)A pep did not develop the disease and presented an increase in IL-10 levels by spleen cells and a decrease in the percentage of CD4+ IFN-γ+ T cells. Moreover, animals receiving the Leader pep presented the highest scores of the disease associated with increase percentage of CD4+ IFN-γ+ T cells. These results would contribute to understanding of the pathogenesis of EAU and support the concept that immune responses to Hsp are of potential importance in exacerbating, perpetuating, or even controlling organ-restricted autoimmune diseases, and it is discussed the irreversibility of autoimmune syndromes. |
format | Online Article Text |
id | pubmed-3433114 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34331142012-09-10 The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis Commodaro, Alessandra Gonçalves Marengo, Eliana Blini Peron, Jean Pierre S. Brandao, Wesley Arslanian, Christina Melo, Robson Lopes Baldon, Estevam J. Belfort, Rubens Sant'Anna, Osvaldo Augusto Rizzo, Luiz Vicente Autoimmune Dis Research Article Experimental autoimmune uveitis (EAU) is a well established model for immune-mediated organ-specific disease. Our group has recently shown that the M. leprae Hsp65 aggravated the uveitis in mice; in the present study, we evaluated the action of M. leprae K(409)A mutant protein and the synthetic peptides Leader pep and K(409)A pep (covering amino acids residues 352–371 of WT and K(409)A proteins of M. leprae Hsp65, resp.) on the pathogenesis of EAU. Mice received the 161–180 IRBP peptide and B. pertussis toxin followed by the intraperitoneal inoculation of K(409)A protein or the Leader pep or K(409)A pep. The Leader pep aggravated the disease, but mice receiving the K(409)A pep did not develop the disease and presented an increase in IL-10 levels by spleen cells and a decrease in the percentage of CD4+ IFN-γ+ T cells. Moreover, animals receiving the Leader pep presented the highest scores of the disease associated with increase percentage of CD4+ IFN-γ+ T cells. These results would contribute to understanding of the pathogenesis of EAU and support the concept that immune responses to Hsp are of potential importance in exacerbating, perpetuating, or even controlling organ-restricted autoimmune diseases, and it is discussed the irreversibility of autoimmune syndromes. Hindawi Publishing Corporation 2012 2012-08-27 /pmc/articles/PMC3433114/ /pubmed/22966424 http://dx.doi.org/10.1155/2012/197648 Text en Copyright © 2012 Alessandra Gonçalves Commodaro et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Commodaro, Alessandra Gonçalves Marengo, Eliana Blini Peron, Jean Pierre S. Brandao, Wesley Arslanian, Christina Melo, Robson Lopes Baldon, Estevam J. Belfort, Rubens Sant'Anna, Osvaldo Augusto Rizzo, Luiz Vicente The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis |
title | The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis |
title_full | The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis |
title_fullStr | The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis |
title_full_unstemmed | The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis |
title_short | The Role of M. leprae Hsp65 Protein and Peptides in the Pathogenesis of Uveitis |
title_sort | role of m. leprae hsp65 protein and peptides in the pathogenesis of uveitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433114/ https://www.ncbi.nlm.nih.gov/pubmed/22966424 http://dx.doi.org/10.1155/2012/197648 |
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