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Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2

Low-power laser irradiation (LPLI) has been found to induce various biological effects and cellular processes. Also, LPLI has been shown to promote fracture repair. Until now, it has been unclear how LPLI promotes bone formation and fracture healing. The aim of this study was to investigate the pote...

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Autores principales: Wu, Jyun-Yi, Wang, Yan-Hsiung, Wang, Gwo-Jaw, Ho, Mei-Ling, Wang, Chau-Zen, Yeh, Ming-Long, Chen, Chia-Hsin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433487/
https://www.ncbi.nlm.nih.gov/pubmed/22962596
http://dx.doi.org/10.1371/journal.pone.0044027
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author Wu, Jyun-Yi
Wang, Yan-Hsiung
Wang, Gwo-Jaw
Ho, Mei-Ling
Wang, Chau-Zen
Yeh, Ming-Long
Chen, Chia-Hsin
author_facet Wu, Jyun-Yi
Wang, Yan-Hsiung
Wang, Gwo-Jaw
Ho, Mei-Ling
Wang, Chau-Zen
Yeh, Ming-Long
Chen, Chia-Hsin
author_sort Wu, Jyun-Yi
collection PubMed
description Low-power laser irradiation (LPLI) has been found to induce various biological effects and cellular processes. Also, LPLI has been shown to promote fracture repair. Until now, it has been unclear how LPLI promotes bone formation and fracture healing. The aim of this study was to investigate the potential mechanism of LPLI-mediated enhancement of bone formation using mouse bone marrow mesenchymal stem cells (D1 cells). D1 cells were irradiated daily with a gallium-aluminum-arsenide (GaAlAs) laser at dose of 0, 1, 2, or 4 J/cm(2). The lactate dehydrogenase (LDH) assay showed no cytotoxic effects of LPLI on D1 cells, and instead, LPLI at 4 J/cm(2) significantly promoted D1 cell proliferation. LPLI also enhanced osteogenic differentiation in a dose-dependent manner and moderately increased expression of osteogenic markers. The neutralization experiments indicated that LPLI regulated insulin-like growth factor 1 (IGF1) and bone morphogenetic protein 2 (BMP2) signaling to promote cell proliferation and/or osteogenic differentiation. In conclusion, our study suggests that LPLI may induce IGF1 expression to promote both the proliferation and osteogenic differentiation of D1 cells, whereas it may induce BMP2 expression primarily to enhance osteogenic differentiation.
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spelling pubmed-34334872012-09-07 Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2 Wu, Jyun-Yi Wang, Yan-Hsiung Wang, Gwo-Jaw Ho, Mei-Ling Wang, Chau-Zen Yeh, Ming-Long Chen, Chia-Hsin PLoS One Research Article Low-power laser irradiation (LPLI) has been found to induce various biological effects and cellular processes. Also, LPLI has been shown to promote fracture repair. Until now, it has been unclear how LPLI promotes bone formation and fracture healing. The aim of this study was to investigate the potential mechanism of LPLI-mediated enhancement of bone formation using mouse bone marrow mesenchymal stem cells (D1 cells). D1 cells were irradiated daily with a gallium-aluminum-arsenide (GaAlAs) laser at dose of 0, 1, 2, or 4 J/cm(2). The lactate dehydrogenase (LDH) assay showed no cytotoxic effects of LPLI on D1 cells, and instead, LPLI at 4 J/cm(2) significantly promoted D1 cell proliferation. LPLI also enhanced osteogenic differentiation in a dose-dependent manner and moderately increased expression of osteogenic markers. The neutralization experiments indicated that LPLI regulated insulin-like growth factor 1 (IGF1) and bone morphogenetic protein 2 (BMP2) signaling to promote cell proliferation and/or osteogenic differentiation. In conclusion, our study suggests that LPLI may induce IGF1 expression to promote both the proliferation and osteogenic differentiation of D1 cells, whereas it may induce BMP2 expression primarily to enhance osteogenic differentiation. Public Library of Science 2012-09-04 /pmc/articles/PMC3433487/ /pubmed/22962596 http://dx.doi.org/10.1371/journal.pone.0044027 Text en © 2012 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wu, Jyun-Yi
Wang, Yan-Hsiung
Wang, Gwo-Jaw
Ho, Mei-Ling
Wang, Chau-Zen
Yeh, Ming-Long
Chen, Chia-Hsin
Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
title Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
title_full Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
title_fullStr Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
title_full_unstemmed Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
title_short Low-Power GaAlAs Laser Irradiation Promotes the Proliferation and Osteogenic Differentiation of Stem Cells via IGF1 and BMP2
title_sort low-power gaalas laser irradiation promotes the proliferation and osteogenic differentiation of stem cells via igf1 and bmp2
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433487/
https://www.ncbi.nlm.nih.gov/pubmed/22962596
http://dx.doi.org/10.1371/journal.pone.0044027
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