An image analysis toolbox for high-throughput C. elegans assays

We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available via the open-source Cel...

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Detalles Bibliográficos
Autores principales: Wählby, Carolina, Kamentsky, Lee, Liu, Zihan H., Riklin-Raviv, Tammy, Conery, Annie L., O’Rourke, Eyleen J., Sokolnicki, Katherine L., Visvikis, Orane, Ljosa, Vebjorn, Irazoqui, Javier E., Golland, Polina, Ruvkun, Gary, Ausubel, Frederick M., Carpenter, Anne E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433711/
https://www.ncbi.nlm.nih.gov/pubmed/22522656
http://dx.doi.org/10.1038/nmeth.1984
Descripción
Sumario:We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems. This WormToolbox is available via the open-source CellProfiler project and enables objective scoring of whole-animal high-throughput image-based assays of C. elegans for the study of diverse biological pathways relevant to human disease.

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