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Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil

BACKGROUND: Porcine circovirus type 2 (PCV2) has been associated with several disease complexes, including reproductive failure. The aim of this study was to identify the subtypes of PCV2 that are associated with reproductive failure in pigs from the State of São Paulo, Brazil and to investigate co-...

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Autores principales: de Castro, Alessandra MMG, Cruz, Taís F, Salgado, Vanessa R, Kanashiro, Tatiana M, Ferrari, Karen L, Araujo, João P, Brandão, Paulo E, Richtzenhain, Leonardo J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434114/
https://www.ncbi.nlm.nih.gov/pubmed/22554105
http://dx.doi.org/10.1186/1751-0147-54-29
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author de Castro, Alessandra MMG
Cruz, Taís F
Salgado, Vanessa R
Kanashiro, Tatiana M
Ferrari, Karen L
Araujo, João P
Brandão, Paulo E
Richtzenhain, Leonardo J
author_facet de Castro, Alessandra MMG
Cruz, Taís F
Salgado, Vanessa R
Kanashiro, Tatiana M
Ferrari, Karen L
Araujo, João P
Brandão, Paulo E
Richtzenhain, Leonardo J
author_sort de Castro, Alessandra MMG
collection PubMed
description BACKGROUND: Porcine circovirus type 2 (PCV2) has been associated with several disease complexes, including reproductive failure. The aim of this study was to identify the subtypes of PCV2 that are associated with reproductive failure in pigs from the State of São Paulo, Brazil and to investigate co-infections with other infectious organisms. FINDINGS: Samples of 168 aborted foetuses or mummified foetuses from five farrow-to-finish swine farms known to be infected with PCV2 and located in the State of São Paulo were tested for PCV2 by polymerase chain reaction (PCR). Positive samples were additionally tested for porcine parvovirus (PPV), Leptospira spp. and Brucella spp. by PCR. PCV2 was detected in 18 of the samples (10.7%). PPV, Brucella spp. and Leptospira spp were found in 2, 10 and 0 cases, respectively. Eleven PCV2 strains were sequenced and determined to be either genotype 2a (n = 1) or 2b (n = 10). CONCLUSIONS: The findings indicate that the frequency of PCV2 infections in aborted porcine foetuses from the State of São Paulo is rather low (10.7%) and that co-infection with other pathogens is common and may be involved in PCV2 associated reproductive failure. No repeatable, characteristic amino acid motifs for regions of the PCV2 capsid protein seemed to be associated with abortion in sows.
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spelling pubmed-34341142012-09-06 Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil de Castro, Alessandra MMG Cruz, Taís F Salgado, Vanessa R Kanashiro, Tatiana M Ferrari, Karen L Araujo, João P Brandão, Paulo E Richtzenhain, Leonardo J Acta Vet Scand Brief Communication BACKGROUND: Porcine circovirus type 2 (PCV2) has been associated with several disease complexes, including reproductive failure. The aim of this study was to identify the subtypes of PCV2 that are associated with reproductive failure in pigs from the State of São Paulo, Brazil and to investigate co-infections with other infectious organisms. FINDINGS: Samples of 168 aborted foetuses or mummified foetuses from five farrow-to-finish swine farms known to be infected with PCV2 and located in the State of São Paulo were tested for PCV2 by polymerase chain reaction (PCR). Positive samples were additionally tested for porcine parvovirus (PPV), Leptospira spp. and Brucella spp. by PCR. PCV2 was detected in 18 of the samples (10.7%). PPV, Brucella spp. and Leptospira spp were found in 2, 10 and 0 cases, respectively. Eleven PCV2 strains were sequenced and determined to be either genotype 2a (n = 1) or 2b (n = 10). CONCLUSIONS: The findings indicate that the frequency of PCV2 infections in aborted porcine foetuses from the State of São Paulo is rather low (10.7%) and that co-infection with other pathogens is common and may be involved in PCV2 associated reproductive failure. No repeatable, characteristic amino acid motifs for regions of the PCV2 capsid protein seemed to be associated with abortion in sows. BioMed Central 2012-05-03 /pmc/articles/PMC3434114/ /pubmed/22554105 http://dx.doi.org/10.1186/1751-0147-54-29 Text en Copyright ©2012 Castro et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
de Castro, Alessandra MMG
Cruz, Taís F
Salgado, Vanessa R
Kanashiro, Tatiana M
Ferrari, Karen L
Araujo, João P
Brandão, Paulo E
Richtzenhain, Leonardo J
Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
title Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
title_full Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
title_fullStr Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
title_full_unstemmed Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
title_short Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of São Paulo, Brazil
title_sort detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the state of são paulo, brazil
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434114/
https://www.ncbi.nlm.nih.gov/pubmed/22554105
http://dx.doi.org/10.1186/1751-0147-54-29
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