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Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor

Adult neurogenesis is restricted to specific brain regions. Although involved in the continuous supply of interneurons for the olfactory function, the role of neural precursors in brain damage-repair remains an open question. Aiming to in vivo identify endogenous neural precursor cells migrating tow...

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Autores principales: Elvira, Gema, García, Isabel, Benito, Marina, Gallo, Juan, Desco, Manuel, Penadés, Soledad, Garcia-Sanz, Jose A., Silva, Augusto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434138/
https://www.ncbi.nlm.nih.gov/pubmed/22957072
http://dx.doi.org/10.1371/journal.pone.0044466
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author Elvira, Gema
García, Isabel
Benito, Marina
Gallo, Juan
Desco, Manuel
Penadés, Soledad
Garcia-Sanz, Jose A.
Silva, Augusto
author_facet Elvira, Gema
García, Isabel
Benito, Marina
Gallo, Juan
Desco, Manuel
Penadés, Soledad
Garcia-Sanz, Jose A.
Silva, Augusto
author_sort Elvira, Gema
collection PubMed
description Adult neurogenesis is restricted to specific brain regions. Although involved in the continuous supply of interneurons for the olfactory function, the role of neural precursors in brain damage-repair remains an open question. Aiming to in vivo identify endogenous neural precursor cells migrating towards a brain damage site, the monoclonal antibody Nilo2 recognizing cell surface antigens on neuroblasts, was coupled to magnetic glyconanoparticles (mGNPs). The Nilo2-mGNP complexes allowed, by magnetic resonance imaging in living animals, the in vivo identification of endogenous neural precursors at their niche, as well as their migration to a lesion site (induced brain tumor), which was fast (within hours) and orderly. Interestingly, the rapid migration of neuroblasts towards a damage site is a characteristic that might be exploited to precisely localize early damage events in neurodegenerative diseases. In addition, it might facilitate the study of regenerative mechanisms through the activation of endogenous neural cell precursors. A similar approach, combining magnetic glyconanoparticles linked to appropriate antibodies could be applied to flag other small cell subpopulations within the organism, track their migration, localize stem cell niches, cancer stem cells or even track metastatic cells.
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spelling pubmed-34341382012-09-06 Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor Elvira, Gema García, Isabel Benito, Marina Gallo, Juan Desco, Manuel Penadés, Soledad Garcia-Sanz, Jose A. Silva, Augusto PLoS One Research Article Adult neurogenesis is restricted to specific brain regions. Although involved in the continuous supply of interneurons for the olfactory function, the role of neural precursors in brain damage-repair remains an open question. Aiming to in vivo identify endogenous neural precursor cells migrating towards a brain damage site, the monoclonal antibody Nilo2 recognizing cell surface antigens on neuroblasts, was coupled to magnetic glyconanoparticles (mGNPs). The Nilo2-mGNP complexes allowed, by magnetic resonance imaging in living animals, the in vivo identification of endogenous neural precursors at their niche, as well as their migration to a lesion site (induced brain tumor), which was fast (within hours) and orderly. Interestingly, the rapid migration of neuroblasts towards a damage site is a characteristic that might be exploited to precisely localize early damage events in neurodegenerative diseases. In addition, it might facilitate the study of regenerative mechanisms through the activation of endogenous neural cell precursors. A similar approach, combining magnetic glyconanoparticles linked to appropriate antibodies could be applied to flag other small cell subpopulations within the organism, track their migration, localize stem cell niches, cancer stem cells or even track metastatic cells. Public Library of Science 2012-09-05 /pmc/articles/PMC3434138/ /pubmed/22957072 http://dx.doi.org/10.1371/journal.pone.0044466 Text en © 2012 Elvira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Elvira, Gema
García, Isabel
Benito, Marina
Gallo, Juan
Desco, Manuel
Penadés, Soledad
Garcia-Sanz, Jose A.
Silva, Augusto
Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor
title Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor
title_full Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor
title_fullStr Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor
title_full_unstemmed Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor
title_short Live Imaging of Mouse Endogenous Neural Progenitors Migrating in Response to an Induced Tumor
title_sort live imaging of mouse endogenous neural progenitors migrating in response to an induced tumor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434138/
https://www.ncbi.nlm.nih.gov/pubmed/22957072
http://dx.doi.org/10.1371/journal.pone.0044466
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