Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases

Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous nucleases may hinder recombination by degrading the exogenous DNA used for reco...

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Detalles Bibliográficos
Autores principales: Mosberg, Joshua A., Gregg, Christopher J., Lajoie, Marc J., Wang, Harris H., Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434165/
https://www.ncbi.nlm.nih.gov/pubmed/22957093
http://dx.doi.org/10.1371/journal.pone.0044638

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434165/
https://www.ncbi.nlm.nih.gov/pubmed/22957093
http://dx.doi.org/10.1371/journal.pone.0044638

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