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Improving Lambda Red Genome Engineering in Escherichia coli via Rational Removal of Endogenous Nucleases
Lambda Red recombineering is a powerful technique for making targeted genetic changes in bacteria. However, many applications are limited by the frequency of recombination. Previous studies have suggested that endogenous nucleases may hinder recombination by degrading the exogenous DNA used for reco...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3434165/ https://www.ncbi.nlm.nih.gov/pubmed/22957093 http://dx.doi.org/10.1371/journal.pone.0044638 |